| http://purl.uniprot.org/citations/21994442 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21994442 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/21994442 | http://www.w3.org/2000/01/rdf-schema#comment | "The highly pathogenic severe acute respiratory syndrome coronavirus (SARS-CoV) poses a constant threat to human health. The viral spike protein (SARS-S) mediates host cell entry and is a potential target for antiviral intervention. Activation of SARS-S by host cell proteases is essential for SARS-CoV infectivity but remains incompletely understood. Here, we analyzed the role of the type II transmembrane serine proteases (TTSPs) human airway trypsin-like protease (HAT) and transmembrane protease, serine 2 (TMPRSS2), in SARS-S activation. We found that HAT activates SARS-S in the context of surrogate systems and authentic SARS-CoV infection and is coexpressed with the viral receptor angiotensin-converting enzyme 2 (ACE2) in bronchial epithelial cells and pneumocytes. HAT cleaved SARS-S at R667, as determined by mutagenesis and mass spectrometry, and activated SARS-S for cell-cell fusion in cis and trans, while the related pulmonary protease TMPRSS2 cleaved SARS-S at multiple sites and activated SARS-S only in trans. However, TMPRSS2 but not HAT expression rendered SARS-S-driven virus-cell fusion independent of cathepsin activity, indicating that HAT and TMPRSS2 activate SARS-S differentially. Collectively, our results show that HAT cleaves and activates SARS-S and might support viral spread in patients."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.05300-11"xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.org/dc/terms/identifier | "doi:10.1128/jvi.05300-11"xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "He Y."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Jahn O."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Lavender H."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Drosten C."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Pohlmann S."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Simmons G."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Glowacka I."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Niemeyer D."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Soilleux E.J."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Muller M.A."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Nehlmeier I."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Bertram S."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Gnirss K."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/author | "Steffen I."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/name | "J Virol"xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/pages | "13363-13372"xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/title | "Cleavage and activation of the severe acute respiratory syndrome coronavirus spike protein by human airway trypsin-like protease."xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://purl.uniprot.org/core/volume | "85"xsd:string |
| http://purl.uniprot.org/citations/21994442 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21994442 |