| http://purl.uniprot.org/citations/21994940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21994940 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21994940 | http://www.w3.org/2000/01/rdf-schema#comment | "Glucocorticoid receptor-α (GRα) and peroxisome proliferator-activated receptor-γ (PPARγ) regulate adipogenesis by controlling the balance between lipolysis and lipogenesis. Here, we show that protein phosphatase 5 (PP5), a nuclear receptor co-chaperone, reciprocally modulates the lipometabolic activities of GRα and PPARγ. Wild-type and PP5-deficient (KO) mouse embryonic fibroblast cells were used to show binding of PP5 to both GRα and PPARγ. In response to adipogenic stimuli, PP5-KO mouse embryonic fibroblast cells showed almost no lipid accumulation with reduced expression of adipogenic markers (aP2, CD36, and perilipin) and low fatty-acid synthase enzymatic activity. This was completely reversed following reintroduction of PP5. Loss of PP5 increased phosphorylation of GRα at serines 212 and 234 and elevated dexamethasone-induced activity at prolipolytic genes. In contrast, PPARγ in PP5-KO cells was hyperphosphorylated at serine 112 but had reduced rosiglitazone-induced activity at lipogenic genes. Expression of the S112A mutant rescued PPARγ transcriptional activity and lipid accumulation in PP5-KO cells pointing to Ser-112 as an important residue of PP5 action. This work identifies PP5 as a fulcrum point in nuclear receptor control of the lipolysis/lipogenesis equilibrium and as a potential target in the treatment of obesity."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.311662"xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.311662"xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Banerjee A."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Banerjee A."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Yong W."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Yong W."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Shou W."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Shou W."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Najjar S.M."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Najjar S.M."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Kaw M.K."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Kaw M.K."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Sanchez E.R."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Sanchez E.R."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Cash H.A."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Cash H.A."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Hinds T.D. Jr."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Hinds T.D. Jr."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Khuder S.S."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Khuder S.S."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Stechschulte L.A."xsd:string |
| http://purl.uniprot.org/citations/21994940 | http://purl.uniprot.org/core/author | "Stechschulte L.A."xsd:string |