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http://purl.uniprot.org/citations/21998677http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21998677http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21998677http://www.w3.org/2000/01/rdf-schema#comment"

Background

MYCT1, a putative target of c-Myc, is a novel candidate tumor suppressor gene cloned from laryngeal squamous cell carcinoma (LSCC). Its transcriptional regulation and biological effects on LSCC have not been clarified.

Methodology/principal findings

Using RACE assay, we cloned a 1106 bp transcript named Myc target 1 transcript variant 1 (MYCT1-TV) and confirmed its transcriptional start site was located at 140 bp upstream of the ATG start codon of MYCT1-TV. Luciferase, electrophoretic mobility shift and chromatin immunoprecipitation assays confirmed c-Myc could regulate the promoter activity of MYCT1-TV by specifically binding to the E-box elements within -886 to -655 bp region. These results were further verified by site-directed mutagenesis and RNA interference (RNAi) assays. MYCT1-TV and MYCT1 expressed lower in LSCC than those in paired adjacent normal laryngeal tissues, and overexpression of MYCT1-TV and MYCT1 could inhibit cell proliferation and invasion and promote apoptosis in LSCC cells.

Conclusions/significance

Our data indicate that MYCT1-TV, a novel MYCT1 transcript, is regulated by c-Myc and down-regulation of MYCT1-TV/MYCT1 could contribute to LSCC development and function."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0025648"xsd:string
http://purl.uniprot.org/citations/21998677http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0025648"xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Chen H."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Chen H."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Guo Y."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Guo Y."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Fu S."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Fu S."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Zhong M."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Zhong M."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Xu Z.M."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Xu Z.M."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Sun K.L."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Sun K.L."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Fu W.N."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Fu W.N."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Qiu G.B."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/author"Qiu G.B."xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/name"PLoS ONE"xsd:string
http://purl.uniprot.org/citations/21998677http://purl.uniprot.org/core/name"PLoS One"xsd:string
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