| http://purl.uniprot.org/citations/22002605 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22002605 | http://www.w3.org/2000/01/rdf-schema#comment | "Repair of DNA double-strand breaks (DSBs) by homologous recombination requires resection of 5'-termini to generate 3'-single-strand DNA tails. Key components of this reaction are exonuclease 1 and the bifunctional endo/exonuclease, Mre11 (refs 2-4). Mre11 endonuclease activity is critical when DSB termini are blocked by bound protein--such as by the DNA end-joining complex, topoisomerases or the meiotic transesterase Spo11 (refs 7-13)--but a specific function for the Mre11 3'-5' exonuclease activity has remained elusive. Here we use Saccharomyces cerevisiae to reveal a role for the Mre11 exonuclease during the resection of Spo11-linked 5'-DNA termini in vivo. We show that the residual resection observed in Exo1-mutant cells is dependent on Mre11, and that both exonuclease activities are required for efficient DSB repair. Previous work has indicated that resection traverses unidirectionally. Using a combination of physical assays for 5'-end processing, our results indicate an alternative mechanism involving bidirectional resection. First, Mre11 nicks the strand to be resected up to 300 nucleotides from the 5'-terminus of the DSB--much further away than previously assumed. Second, this nick enables resection in a bidirectional manner, using Exo1 in the 5'-3' direction away from the DSB, and Mre11 in the 3'-5' direction towards the DSB end. Mre11 exonuclease activity also confers resistance to DNA damage in cycling cells, suggesting that Mre11-catalysed resection may be a general feature of various DNA repair pathways."xsd:string |
| http://purl.uniprot.org/citations/22002605 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature10515"xsd:string |
| http://purl.uniprot.org/citations/22002605 | http://purl.uniprot.org/core/author | "Garcia V."xsd:string |
| http://purl.uniprot.org/citations/22002605 | http://purl.uniprot.org/core/author | "Gray S."xsd:string |
| http://purl.uniprot.org/citations/22002605 | http://purl.uniprot.org/core/author | "Neale M.J."xsd:string |
| http://purl.uniprot.org/citations/22002605 | http://purl.uniprot.org/core/author | "Phelps S.E."xsd:string |
| http://purl.uniprot.org/citations/22002605 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/22002605 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/22002605 | http://purl.uniprot.org/core/pages | "241-244"xsd:string |
| http://purl.uniprot.org/citations/22002605 | http://purl.uniprot.org/core/title | "Bidirectional resection of DNA double-strand breaks by Mre11 and Exo1."xsd:string |
| http://purl.uniprot.org/citations/22002605 | http://purl.uniprot.org/core/volume | "479"xsd:string |
| http://purl.uniprot.org/citations/22002605 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22002605 |
| http://purl.uniprot.org/citations/22002605 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22002605 |
| http://purl.uniprot.org/uniprot/P32829#attribution-A492F160D800F664A675D684ABEC7D42 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22002605 |
| http://purl.uniprot.org/uniprot/#_P20050-mappedCitation-22002605 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22002605 |
| http://purl.uniprot.org/uniprot/#_P23179-mappedCitation-22002605 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22002605 |
| http://purl.uniprot.org/uniprot/#_P38110-mappedCitation-22002605 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22002605 |
| http://purl.uniprot.org/uniprot/#_P39875-mappedCitation-22002605 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22002605 |
| http://purl.uniprot.org/uniprot/#_P32829-mappedCitation-22002605 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22002605 |
| http://purl.uniprot.org/uniprot/P39875 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22002605 |
| http://purl.uniprot.org/uniprot/P32829 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22002605 |
| http://purl.uniprot.org/uniprot/P38110 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22002605 |
| http://purl.uniprot.org/uniprot/P20050 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22002605 |
| http://purl.uniprot.org/uniprot/P23179 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22002605 |