| http://purl.uniprot.org/citations/22007023 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22007023 | http://www.w3.org/2000/01/rdf-schema#comment | "The Arabidopsis (Arabidopsis thaliana) phytoalexin-deficient mutant pad2-1 displays enhanced susceptibility to a broad range of pathogens and herbivorous insects that correlates with deficiencies in the production of camalexin, indole glucosinolates, and salicylic acid (SA). The pad2-1 mutation is localized in the GLUTAMATE-CYSTEINE LIGASE (GCL) gene encoding the first enzyme of glutathione biosynthesis. While pad2-1 glutathione deficiency is not caused by a decrease in GCL transcripts, analysis of GCL protein level revealed that pad2-1 plants contained only 48% of the wild-type protein amount. In contrast to the wild type, the oxidized form of GCL was dominant in pad2-1, suggesting a distinct redox environment. This finding was corroborated by the expression of GRX1-roGFP2, showing that the cytosolic glutathione redox potential was significantly less negative in pad2-1. Analysis of oxidative stress-related gene expression showed a higher transcript accumulation in pad2-1 of GLUTATHIONE REDUCTASE, GLUTATHIONE-S-TRANSFERASE, and RESPIRATORY BURST OXIDASE HOMOLOG D in response to the oomycete Phytophthora brassicae. Interestingly, oligogalacturonide elicitation in pad2-1 revealed a lower plasma membrane depolarization that was found to act upstream of an impaired hydrogen peroxide production. This impaired hydrogen peroxide production was also observed during pathogen infection and correlated with a reduced hypersensitive response in pad2-1. In addition, a lack of pathogen-triggered expression of the ISOCHORISMATE SYNTHASE1 gene, coding for the SA-biosynthetic enzyme isochorismate synthase, was identified as the cause of the SA deficiency in pad2-1. Together, our results indicate that the pad2-1 mutation is related to a decrease in GCL protein and that the resulting glutathione deficiency negatively affects important processes of disease resistance."xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.111.182667"xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/author | "Mauch F."xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/author | "Meyer A.J."xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/author | "Frettinger P."xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/author | "Poinssot B."xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/author | "Wendehenne D."xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/author | "Marty L."xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/author | "Vitecek J."xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/author | "Dubreuil-Maurizi C."xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/author | "Branciard L."xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/name | "Plant Physiol"xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/pages | "2000-2012"xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/title | "Glutathione deficiency of the Arabidopsis mutant pad2-1 affects oxidative stress-related events, defense gene expression, and the hypersensitive response."xsd:string |
| http://purl.uniprot.org/citations/22007023 | http://purl.uniprot.org/core/volume | "157"xsd:string |
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