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| http://purl.uniprot.org/citations/22010828 | http://www.w3.org/2000/01/rdf-schema#comment | "A yeast two-hybrid screen performed to identify binding partners of the CaR (calcium-sensing receptor) intracellular tail identified the adaptor protein 14-3-3θ as a novel binding partner that bound to the proximal membrane region important for CaR expression and signalling. The 14-3-3θ protein directly interacted with the CaR tail in pull-down studies and FLAG-tagged CaR co-immunoprecipitated with EGFP (enhanced green fluorescent protein)-tagged 14-3-3θ when co-expressed in HEK (human embryonic kidney)-293 or COS-1 cells. The interaction between the CaR and 14-3-3θ did not require a putative binding site in the membrane-proximal region of the CaR tail and was independent of PKC (protein kinase C) phosphorylation. Confocal microscopy demonstrated co-localization of the CaR and EGFP-14-3-3θ in the ER (endoplasmic reticulum) of HEK-293 cells that stably expressed the CaR (HEK-293/CaR cells), but 14-3-3θ overexpression had no effect on membrane expression of the CaR. Overexpression of 14-3-3θ in HEK-293/CaR cells attenuated CaR-mediated Rho signalling, but had no effect on ERK (extracellular-signal-regulated kinase) 1/2 signalling. Another isoform identified from the library, 14-3-3ζ, exhibited similar behaviour to that of 14-3-3θ with respect to CaR tail binding, cellular co-localization and impact on receptor-mediated signalling. However, unlike 14-3-3θ, this isoform, when overexpressed, significantly reduced CaR plasma membrane expression. Results indicate that 14-3-3 proteins mediate CaR-dependent Rho signalling and may modulate the plasma membrane expression of the CaR."xsd:string |
| http://purl.uniprot.org/citations/22010828 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj20111277"xsd:string |
| http://purl.uniprot.org/citations/22010828 | http://purl.uniprot.org/core/author | "Ingley E."xsd:string |
| http://purl.uniprot.org/citations/22010828 | http://purl.uniprot.org/core/author | "Brown S.J."xsd:string |
| http://purl.uniprot.org/citations/22010828 | http://purl.uniprot.org/core/author | "Conigrave A.D."xsd:string |
| http://purl.uniprot.org/citations/22010828 | http://purl.uniprot.org/core/author | "Ratajczak T."xsd:string |
| http://purl.uniprot.org/citations/22010828 | http://purl.uniprot.org/core/author | "Ward B.K."xsd:string |
| http://purl.uniprot.org/citations/22010828 | http://purl.uniprot.org/core/author | "Arulpragasam A."xsd:string |
| http://purl.uniprot.org/citations/22010828 | http://purl.uniprot.org/core/author | "Magno A.L."xsd:string |
| http://purl.uniprot.org/citations/22010828 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22010828 | http://purl.uniprot.org/core/name | "Biochem J"xsd:string |
| http://purl.uniprot.org/citations/22010828 | http://purl.uniprot.org/core/pages | "995-1006"xsd:string |
| http://purl.uniprot.org/citations/22010828 | http://purl.uniprot.org/core/title | "The adaptor protein 14-3-3 binds to the calcium-sensing receptor and attenuates receptor-mediated Rho kinase signalling."xsd:string |
| http://purl.uniprot.org/citations/22010828 | http://purl.uniprot.org/core/volume | "441"xsd:string |
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