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http://purl.uniprot.org/citations/22024089http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Background/aims

This study aimed to investigate the effects of yes-associated protein (YAP) silencing on invasion, migration, anchorage-independent growth and angiogenesis in the human gastric cancer cell line SGC-7901.

Methodology

SGC-7901 cells were transfected with a shRNA lentivirus vector targeting YAP gene. After puromycin selection of stable YAP-silenced SGC-7901 cell lines, the expression of YAP mRNA and protein were detected using quantitative real-time PCR (qRT-PCR) and western blotting. A transwell invasion assay, wound healing assay and colony formation assay were used to analyze the invasion, migration and anchorage-independent growth effect of RNA interference (RNAi) on YAP expression. The angiogenic cytokines, vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF-2), were measured by qRT-PCR and western blotting.

Results

The results of the qRT-PCR and western blotting showed an obvious inhibition of YAP mRNA and protein expression in the YAP-shRNA group. Moreover, the inhibition of YAP expression clearly impeded SGC-7901 cell migration, invasion and anchorage-independent growth. Furthermore, VEGF and FGF-2 were also downregulated.

Conclusions

YAP gene targeting by RNAi can inhibit the migration, invasion, anchorage-independent growth and angiogenesis ability of SGC-7901 cells. YAP may be a potential target for the treatment of human gastric cancer."xsd:string
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http://purl.uniprot.org/citations/22024089http://purl.uniprot.org/core/author"Zhou Z."xsd:string
http://purl.uniprot.org/citations/22024089http://purl.uniprot.org/core/author"Xu Z.P."xsd:string
http://purl.uniprot.org/citations/22024089http://purl.uniprot.org/core/author"Zhu J.S."xsd:string
http://purl.uniprot.org/citations/22024089http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/22024089http://purl.uniprot.org/core/name"Hepatogastroenterology"xsd:string
http://purl.uniprot.org/citations/22024089http://purl.uniprot.org/core/pages"2156-2161"xsd:string
http://purl.uniprot.org/citations/22024089http://purl.uniprot.org/core/title"RNA interference mediated YAP gene silencing inhibits invasion and metastasis of human gastric cancer cell line SGC-7901."xsd:string
http://purl.uniprot.org/citations/22024089http://purl.uniprot.org/core/volume"58"xsd:string
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