| http://purl.uniprot.org/citations/22040088 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22040088 | http://www.w3.org/2000/01/rdf-schema#comment | "Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen that possesses a type III secretion system (T3SS) critical for evading innate immunity and establishing acute infections in compromised patients. Our research has focused on the structure-activity relationships of ExoU, the most toxic and destructive type III effector produced by P. aeruginosa. ExoU possesses phospholipase activity, which is detectable in vitro only when a eukaryotic cofactor is provided with membrane substrates. We report here that a subpopulation of ubiquitylated yeast SOD1 and other ubiquitylated mammalian proteins activate ExoU. Phospholipase activity was detected using purified ubiquitin of various chain lengths and linkage types; however, free monoubiquitin is sufficient in a genetically engineered dual expression system. The use of ubiquitin by a bacterial enzyme as an activator is unprecedented and represents a new aspect in the manipulation of the eukaryotic ubiquitin system to facilitate bacterial replication and dissemination."xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2958.2011.07904.x"xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/author | "Sato H."xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/author | "Terhune S.S."xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/author | "Anderson D.M."xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/author | "Frank D.W."xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/author | "Casey M."xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/author | "Haas A.L."xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/author | "Feix J.B."xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/author | "Schmalzer K.M."xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/name | "Mol Microbiol"xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/pages | "1454-1467"xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/title | "Ubiquitin and ubiquitin-modified proteins activate the Pseudomonas aeruginosa T3SS cytotoxin, ExoU."xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://purl.uniprot.org/core/volume | "82"xsd:string |
| http://purl.uniprot.org/citations/22040088 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22040088 |
| http://purl.uniprot.org/citations/22040088 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22040088 |
| http://purl.uniprot.org/uniprot/#_P00445-mappedCitation-22040088 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22040088 |
| http://purl.uniprot.org/uniprot/P00445 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22040088 |