| http://purl.uniprot.org/citations/22045333 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22045333 | http://www.w3.org/2000/01/rdf-schema#comment | "Posttranscriptional gene silencing is mediated by RNA-induced silencing complexes (RISCs) that contain AGO proteins and single-stranded small RNAs. The assembly of plant AGO1-containing RISCs depends on the molecular chaperone HSP90. Here, we demonstrate that cyclophilin 40 (CYP40), protein phosphatase 5 (PP5), and several other proteins with the tetratricopeptide repeat (TPR) domain associates with AGO1 in an HSP90-dependent manner in extracts of evacuolated tobacco protoplasts (BYL). Intriguingly, CYP40, but not the other TPR proteins, could form a complex with small RNA duplex-bound AGO1. Moreover, CYP40 that was synthesized by in-vitro translation using BYL uniquely facilitated binding of small RNA duplexes to AGO1, and as a result, increased the amount of mature RISCs that could cleave target RNAs. CYP40 was not contained in mature RISCs, indicating that the association is transient. Addition of PP5 or cyclophilin-binding drug cyclosporine A prevented the association of endogenous CYP40 with HSP90-AGO1 complex and inhibited RISC assembly. These results suggest that a complex of AGO1, HSP90, CYP40, and a small RNA duplex is a key intermediate of RISC assembly in plants."xsd:string |
| http://purl.uniprot.org/citations/22045333 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2011.395"xsd:string |
| http://purl.uniprot.org/citations/22045333 | http://purl.uniprot.org/core/author | "Yoshikawa M."xsd:string |
| http://purl.uniprot.org/citations/22045333 | http://purl.uniprot.org/core/author | "Meshi T."xsd:string |
| http://purl.uniprot.org/citations/22045333 | http://purl.uniprot.org/core/author | "Ishikawa M."xsd:string |
| http://purl.uniprot.org/citations/22045333 | http://purl.uniprot.org/core/author | "Iki T."xsd:string |
| http://purl.uniprot.org/citations/22045333 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22045333 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/22045333 | http://purl.uniprot.org/core/pages | "267-278"xsd:string |
| http://purl.uniprot.org/citations/22045333 | http://purl.uniprot.org/core/title | "Cyclophilin 40 facilitates HSP90-mediated RISC assembly in plants."xsd:string |
| http://purl.uniprot.org/citations/22045333 | http://purl.uniprot.org/core/volume | "31"xsd:string |
| http://purl.uniprot.org/citations/22045333 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22045333 |
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