| http://purl.uniprot.org/citations/22053215 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22053215 | http://www.w3.org/2000/01/rdf-schema#comment | "Synthetic oligodeoxynucleotides containing unmethylated CpG motifs (CpG) stimulate innate immune responses. Phosphoinositide 3-kinase (PI3K) has been implicated in CpG-induced immune activation; however, its precise role has not yet been clarified. CpG-induced production of IL-10 was dramatically increased in macrophages deficient in PI3Kγ (p110γ(-/-)). By contrast, LPS-induced production of IL-10 was unchanged in the cells. CpG-induced, but not LPS-induced, IL-10 production was almost completely abolished in SCID mice having mutations in DNA-dependent protein kinase catalytic subunit (DNA-PKcs). Furthermore, wortmannin, an inhibitor of DNA-PKcs, completely inhibited CpG-induced IL-10 production, both in wild type and p110γ(-/-) cells. Microscopic analyses revealed that CpG preferentially localized with DNA-PKcs in p110γ(-/-) cells than in wild type cells. In addition, CpG was preferentially co-localized with the acidic lysosomal marker, LysoTracker, in p110γ(-/-) cells, and with an early endosome marker, EEA1, in wild type cells. Over-expression of p110γ in Cos7 cells resulted in decreased acidification of CpG containing endosome. A similar effect was reproduced using kinase-dead mutants, but not with a ras-binding site mutant, of p110γ. Thus, it is likely that p110γ, in a manner independent of its kinase activity, inhibits the acidification of CpG-containing endosomes. It is considered that increased acidification of CpG-containing endosomes in p110γ(-/-) cells enforces endosomal escape of CpG, which results in increased association of CpG with DNA-PKcs to up-regulate IL-10 production in macrophages."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0026836"xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/author | "Ishikawa Y."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/author | "Matsumoto M."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/author | "Murakami H."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/author | "Sasaki T."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/author | "Seya T."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/author | "Uehara M."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/author | "Takasuga S."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/author | "Kametani Y."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/author | "Hazeki K."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/author | "Hazeki O."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/author | "Nigorikawa K."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/pages | "e26836"xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/title | "Phosphoinositide 3-kinasegamma controls the intracellular localization of CpG to limit DNA-PKcs-dependent IL-10 production in macrophages."xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/22053215 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22053215 |
| http://purl.uniprot.org/citations/22053215 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22053215 |
| http://purl.uniprot.org/uniprot/#_A0A087WQM2-mappedCitation-22053215 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22053215 |
| http://purl.uniprot.org/uniprot/#_A0A7R8GUQ3-mappedCitation-22053215 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22053215 |
| http://purl.uniprot.org/uniprot/#_A0A1W2P8F6-mappedCitation-22053215 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22053215 |
| http://purl.uniprot.org/uniprot/#_Q24LN5-mappedCitation-22053215 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22053215 |