| http://purl.uniprot.org/citations/22074159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22074159 | http://www.w3.org/2000/01/rdf-schema#comment | "Quantitative mass spectrometry (MS) in combination with affinity purification approaches allows for an unbiased study of protein-protein and peptide-protein interactions. In shotgun approaches that are based on proteolytic digestion of complex protein mixtures followed by two-dimensional liquid-phase chromatography, the separation effort prior to MS analysis is focused on tryptic peptides. Here we developed an improved offline 2-D liquid chromatography-MS/MS approach for the identification and quantification of binding proteins utilizing reversed-phase capillary columns with acidic acetonitrile-containing eluents in both chromatographic dimensions. A specific fractionation scheme was applied in order to obtain samples with evenly distributed peptides and to fully utilize the separation space in the second dimension nanoLC-MS/MS. We report peptide-protein interaction studies to identify phosphorylation-dependent binding partners of the T cell adapter protein ADAP. The results of the SILAC-based pull-down experiments show this approach is well suited for distinguishing phosphorylation-specific interactions from unspecific binding events. The data provide further evidence that phosphorylated Tyr 595 of ADAP may serve as a direct binding site for the SH2 domains of the T cell proteins SLP76 and NCK. From a technical point of view we provide a detailed protocol for an offline 2-D RP-RP LC-MS/MS method that offers a robust and time-saving alternative for quantitative interactome analysis."xsd:string |
| http://purl.uniprot.org/citations/22074159 | http://purl.org/dc/terms/identifier | "doi:10.1021/pr200900s"xsd:string |
| http://purl.uniprot.org/citations/22074159 | http://purl.uniprot.org/core/author | "Lang D."xsd:string |
| http://purl.uniprot.org/citations/22074159 | http://purl.uniprot.org/core/author | "Lange S."xsd:string |
| http://purl.uniprot.org/citations/22074159 | http://purl.uniprot.org/core/author | "Krause E."xsd:string |
| http://purl.uniprot.org/citations/22074159 | http://purl.uniprot.org/core/author | "Freund C."xsd:string |
| http://purl.uniprot.org/citations/22074159 | http://purl.uniprot.org/core/author | "Stephanowitz H."xsd:string |
| http://purl.uniprot.org/citations/22074159 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22074159 | http://purl.uniprot.org/core/name | "J Proteome Res"xsd:string |
| http://purl.uniprot.org/citations/22074159 | http://purl.uniprot.org/core/pages | "1175-1183"xsd:string |
| http://purl.uniprot.org/citations/22074159 | http://purl.uniprot.org/core/title | "Improved two-dimensional reversed phase-reversed phase LC-MS/MS approach for identification of peptide-protein interactions."xsd:string |
| http://purl.uniprot.org/citations/22074159 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/22074159 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22074159 |
| http://purl.uniprot.org/citations/22074159 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22074159 |
| http://purl.uniprot.org/uniprot/#_O15117-mappedCitation-22074159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22074159 |
| http://purl.uniprot.org/uniprot/#_P27986-mappedCitation-22074159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22074159 |
| http://purl.uniprot.org/uniprot/#_Q13094-mappedCitation-22074159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22074159 |
| http://purl.uniprot.org/uniprot/#_P16333-mappedCitation-22074159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22074159 |
| http://purl.uniprot.org/uniprot/#_P16591-mappedCitation-22074159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22074159 |
| http://purl.uniprot.org/uniprot/#_O75791-mappedCitation-22074159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22074159 |
| http://purl.uniprot.org/uniprot/#_P46108-mappedCitation-22074159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22074159 |
| http://purl.uniprot.org/uniprot/#_P46109-mappedCitation-22074159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22074159 |
| http://purl.uniprot.org/uniprot/#_O43639-mappedCitation-22074159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22074159 |
| http://purl.uniprot.org/uniprot/#_P19174-mappedCitation-22074159 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22074159 |