| http://purl.uniprot.org/citations/22074260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22074260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22074260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/22074260 | http://www.w3.org/2000/01/rdf-schema#comment | "We discovered that the PF1549 gene in Pyrococcus furiosus encodes a very heat-stable RNA 3'-terminal phosphate cyclase (Pf-Rtc). Although all previously reported Rtc proteins are ATP-dependent enzymes, we found that Pf-Rtc requires GTP for its cyclase activity at 95 °C. Low-level activation of the enzyme was also observed in the presence of dGTP but not other dNTPs, indicating that the guanine base is very important for Pf-Rtc activity. We analyzed a series of GTP analogues and found that the conversion from GTP to GMP is important for Pf-Rtc activity and that an excess of GMP inhibits this activity. Gel-shift analysis clearly showed that the RNA-binding activity of Pf-Rtc is totally dependent on the linear form of the 3'-terminal phosphate, with an apparent K(d) value of 20 nm at 95°C. Furthermore, we found that Pf-Rtc may contribute to GTP-dependent RNA ligation activity through the PF0027 protein (a 2'-5' RNA ligase-like protein in P. furiosus). The possible roles of Pf-Rtc and the importance of terminal phosphate structures in RNA are discussed."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2443.2011.01561.x"xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2443.2011.01561.x"xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1365-2443.2011.01561.x"xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/author | "Igarashi K."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/author | "Igarashi K."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/author | "Kanai A."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/author | "Kanai A."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/author | "Sato A."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/author | "Sato A."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/author | "Tomita M."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/author | "Tomita M."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/author | "Soga T."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/author | "Soga T."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/author | "Takesue K."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/author | "Takesue K."xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/name | "Genes Cells"xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/name | "Genes Cells"xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/pages | "1190-1199"xsd:string |
| http://purl.uniprot.org/citations/22074260 | http://purl.uniprot.org/core/pages | "1190-1199"xsd:string |