| http://purl.uniprot.org/citations/22085930 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22085930 | http://www.w3.org/2000/01/rdf-schema#comment | "Ubiquitin-conjugating enzymes (E2s) coordinate distinct types of ubiquitination via specific E3 ligases, to a large number of protein substrates. While many E2 enzymes need only the presence of an E3 ligase for substrate ubiquitination, a number of E2s require additional, non-canonical binding partners to specify their function. Here, we have determined the crystal structure and function of an E2/co-activator assembly, the Pex4p:Pex22p complex. The peroxisome-associated E2 enzyme Pex4p binds the peroxisomal membrane protein Pex22p through a binding site that does not overlap with any other known interaction interface in E2 enzymes. Pex22p association enhances Pex4p's ability to transfer ubiquitin to a substrate in vitro, and Pex22p binding-deficient forms of Pex4p are unable to ubiquitinate the peroxisomal import receptor Pex5p in vivo. Our data demonstrate that the Pex4p:Pex22p assembly, and not Pex4p alone, functions as the E2 enzyme required for Pex5p ubiquitination, establishing a novel mechanism of E2 enzyme regulation."xsd:string |
| http://purl.uniprot.org/citations/22085930 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2011.411"xsd:string |
| http://purl.uniprot.org/citations/22085930 | http://purl.uniprot.org/core/author | "Wilmanns M."xsd:string |
| http://purl.uniprot.org/citations/22085930 | http://purl.uniprot.org/core/author | "Panjikar S."xsd:string |
| http://purl.uniprot.org/citations/22085930 | http://purl.uniprot.org/core/author | "Williams C."xsd:string |
| http://purl.uniprot.org/citations/22085930 | http://purl.uniprot.org/core/author | "van den Berg M."xsd:string |
| http://purl.uniprot.org/citations/22085930 | http://purl.uniprot.org/core/author | "Distel B."xsd:string |
| http://purl.uniprot.org/citations/22085930 | http://purl.uniprot.org/core/author | "Stanley W.A."xsd:string |
| http://purl.uniprot.org/citations/22085930 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22085930 | http://purl.uniprot.org/core/name | "EMBO J"xsd:string |
| http://purl.uniprot.org/citations/22085930 | http://purl.uniprot.org/core/pages | "391-402"xsd:string |
| http://purl.uniprot.org/citations/22085930 | http://purl.uniprot.org/core/title | "Insights into ubiquitin-conjugating enzyme/ co-activator interactions from the structure of the Pex4p:Pex22p complex."xsd:string |
| http://purl.uniprot.org/citations/22085930 | http://purl.uniprot.org/core/volume | "31"xsd:string |
| http://purl.uniprot.org/citations/22085930 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22085930 |
| http://purl.uniprot.org/citations/22085930 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22085930 |
| http://purl.uniprot.org/uniprot/P39718#attribution-5C046D23204BC978F81382BDB1A52116 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22085930 |
| http://purl.uniprot.org/uniprot/P29340#attribution-5C046D23204BC978F81382BDB1A52116 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22085930 |
| http://purl.uniprot.org/uniprot/#_A0A8H4FBM1-mappedCitation-22085930 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22085930 |
| http://purl.uniprot.org/uniprot/#_A0A6V8S9F1-mappedCitation-22085930 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22085930 |
| http://purl.uniprot.org/uniprot/#_P39718-mappedCitation-22085930 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22085930 |
| http://purl.uniprot.org/uniprot/#_P29340-mappedCitation-22085930 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22085930 |
| http://purl.uniprot.org/uniprot/A0A6V8S9F1 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22085930 |
| http://purl.uniprot.org/uniprot/P39718 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22085930 |
| http://purl.uniprot.org/uniprot/A0A8H4FBM1 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22085930 |