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http://purl.uniprot.org/citations/22093310http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Objective

To understand the role of heme oxygenase-1 (HO-1) in hydrogen peroxide [H(2)O(2)] induced apoptosis and mitochondrial trans-membrane potential (MTMP) change in primary alveolar epithelial cell type II(AEC II).

Methods

Primary AEC II collected from healthy Sprague Dawley (SD) rats were cultured for 24 hours, then divided into four groups to be treated with: (1) saline; (2) H(2)O(2) (0.5 mmol/L); (3) H(2)O(2) +HO-1 (0.2 mmol/L); (4) H(2)O(2) +zinc original porphyrin IX (HO-1 inhibitor, 20 μmol/L). The morphology of cells in the cultures was examined by fluorescent microscopy 2.5 hours later, and the number of apoptotic cells / the MTMP determined by flow-cytometry 0.5, 1.0, 1.5, 2.0 and 2.5 hours later.

Results

Large number of cells in with green (early apoptotic) or red (later apoptotic) fluorescence were observed by microscope in cultures treated with H(2)O(2) , and H(2)O(2) + HO-1 inhibitor, but such cells were obviously fewer in HO-1 treated cultures. Compared with saline treated cells, H(2)O(2) treated cells had significantly higher apoptosis rate, that increased with time, reaching peak value 2.5 hours into the treatment [0.5 hour: (30.27 ± 0.74)% vs. (3.76 ± 0.81)%, 2.5 hours: (40.46 ± 0.91)% vs. (22.74 ± 0.60)%, both P < 0.05], while the rate of MTMP depolarization was significantly lower (0.99 ± 0.21 vs. 1.91 ± 0.16, P < 0.05) in these cells. Compared with H(2)O(2) treated cells, the apoptosis rate in HO-1 treated cells was significantly lower [0.5 hour: (5.99 ± 0.60)% vs. (30.27 ± 0.74)%, 2.5 hours: (22.69 ± 1.69)% vs. (40.46 ± 0.91)%, both P < 0.05], and their rate of MTMP depolarization higher (2.02 ± 0.12 vs. 0.99 ± 0.21, P < 0.05). Compared with HO-1 treated cells, HO-1 inhibitor treated cells had significantly higher apoptosis rate which reached peak value 2.5 hours into the treatment [0.5 hour: (30.73 ± 1.08)% vs. (5.99 ± 0.60)%, 2.5 hours: (41.38 ± 0.57)% vs. (22.69 ± 1.69)%, both P < 0.05], while rate of MTMP depolarization in these cells was significantly lower (0.98 ± 0.09 vs. 2.02 ± 0.12, P < 0.05).

Conclusion

HO-1 could maintain the integrity of AEC II and stabilize their mitochondria membrane potential, protecting the cells from H(2)O(2) induced damage."xsd:string
http://purl.uniprot.org/citations/22093310http://purl.uniprot.org/core/author"Chen M."xsd:string
http://purl.uniprot.org/citations/22093310http://purl.uniprot.org/core/author"Wu Y."xsd:string
http://purl.uniprot.org/citations/22093310http://purl.uniprot.org/core/author"Wu S."xsd:string
http://purl.uniprot.org/citations/22093310http://purl.uniprot.org/core/author"Wang Y.H."xsd:string
http://purl.uniprot.org/citations/22093310http://purl.uniprot.org/core/author"Wang H.M."xsd:string
http://purl.uniprot.org/citations/22093310http://purl.uniprot.org/core/author"Fu X.Y."xsd:string
http://purl.uniprot.org/citations/22093310http://purl.uniprot.org/core/author"Qian M.J."xsd:string
http://purl.uniprot.org/citations/22093310http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/22093310http://purl.uniprot.org/core/name"Zhongguo Wei Zhong Bing Ji Jiu Yi Xue"xsd:string
http://purl.uniprot.org/citations/22093310http://purl.uniprot.org/core/pages"658-660"xsd:string
http://purl.uniprot.org/citations/22093310http://purl.uniprot.org/core/title"[The role of heme oxygenase 1 in hydrogen peroxide induced apoptosis and mitochondrial trans membrane potential change in rat primary type II alveolar epithelium cells]."xsd:string
http://purl.uniprot.org/citations/22093310http://purl.uniprot.org/core/volume"23"xsd:string
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