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http://purl.uniprot.org/citations/22093778http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
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Objective

To investigate the expression of tumor necrosis factor-alpha (TNF-α) and cyclooxygenase-2 (COX-2) mRNA and evaluate the status of histone H3 acetylation at TNF-α and COX-2 promoter in peripheral blood mononuclear cells (PBMCs) from type 2 diabetics.

Methods

The PBMCs from 12 type 2 diabetics and 12 healthy controls were isolated by Ficoll-Hypaque density gradient centrifugation. The differential expression of TNF-α and COX-2 mRNA was measured by real-time PCR (polymerase chain reaction). Chromatin immunoprecipitation analysis was used to detect the status of H3 acetylation at TNF-α and COX-2 promoter region.

Results

TNF-α and COX-2 mRNA were overexpressed in PBMCs from Type 2 diabetics as compared with normal controls (2.28 ± 0.09 fold and 2.78 ± 0.26 fold).(P < 0.05). Compared with normal controls, H3 acetylation at the TNF-α (1.54 ± 0.43 vs 0.97 ± 0.39, P = 0.0094) and COX-2 (1.20 ± 0.58 vs 0.64 ± 0.21, P = 0.0161) gene promoter region was elevated in PBMCs from Type 2 diabetic patients.

Conclusion

Increased H3 acetylation at TNF-α and COX-2 promoter in PBMCs from type 2 diabetics may contribute to the pathogenesis of type 2 diabetes through the elevated expressions of TNF-α and COX-2."xsd:string
http://purl.uniprot.org/citations/22093778http://purl.uniprot.org/core/author"Li X."xsd:string
http://purl.uniprot.org/citations/22093778http://purl.uniprot.org/core/author"Lin Y."xsd:string
http://purl.uniprot.org/citations/22093778http://purl.uniprot.org/core/author"Zhao M."xsd:string
http://purl.uniprot.org/citations/22093778http://purl.uniprot.org/core/author"Hou C."xsd:string
http://purl.uniprot.org/citations/22093778http://purl.uniprot.org/core/author"Li Y.J."xsd:string
http://purl.uniprot.org/citations/22093778http://purl.uniprot.org/core/author"Zhou Z.G."xsd:string
http://purl.uniprot.org/citations/22093778http://purl.uniprot.org/core/author"Lu Q.J."xsd:string
http://purl.uniprot.org/citations/22093778http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/22093778http://purl.uniprot.org/core/name"Zhonghua Yi Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/22093778http://purl.uniprot.org/core/pages"1805-1808"xsd:string
http://purl.uniprot.org/citations/22093778http://purl.uniprot.org/core/title"[Histone H3 acetylation of tumor necrosis factor-alpha and cyclooxygenase-2 in patients with type 2 diabetes]."xsd:string
http://purl.uniprot.org/citations/22093778http://purl.uniprot.org/core/volume"91"xsd:string
http://purl.uniprot.org/citations/22093778http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/22093778
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