| http://purl.uniprot.org/citations/22102253 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22102253 | http://www.w3.org/2000/01/rdf-schema#comment | "The L27 (LIN-2/LIN-7) domain is a protein-protein interaction module capable of assembling proteins into biologically important complexes. Pals1 contains two L27 domains: the first, L27N, interacts with PATJ, and the second, L27C, interacts with MALS, forming a tripartite complex that plays a crucial role in the establishment and maintenance of cell polarity. To provide a better understanding of the mechanism of assembly of this tripartite complex, four different L27(PATJ)-(L27N,L27C)(Pals1)-L27(MALS) constructs were cloned, expressed, purified and crystallized. Crystals of tripartite complex 1 of L27(PATJ)-(L27N,L27C)(Pals1)-L27(MALS) diffracted to 2.05 Å resolution. These crystals belonged to either space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 145.2, c = 202.5 Å. Assuming the presence of four molecules in the asymmetric unit, a Matthews coefficient of 2.69 Å(3) Da(-1) was calculated, corresponding to a solvent content of 54.25%."xsd:string |
| http://purl.uniprot.org/citations/22102253 | http://purl.org/dc/terms/identifier | "doi:10.1107/s174430911103689x"xsd:string |
| http://purl.uniprot.org/citations/22102253 | http://purl.uniprot.org/core/author | "Shen Y."xsd:string |
| http://purl.uniprot.org/citations/22102253 | http://purl.uniprot.org/core/author | "Yang X."xsd:string |
| http://purl.uniprot.org/citations/22102253 | http://purl.uniprot.org/core/author | "Zhang J."xsd:string |
| http://purl.uniprot.org/citations/22102253 | http://purl.uniprot.org/core/author | "Long J."xsd:string |
| http://purl.uniprot.org/citations/22102253 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/22102253 | http://purl.uniprot.org/core/name | "Acta Crystallogr Sect F Struct Biol Cryst Commun"xsd:string |
| http://purl.uniprot.org/citations/22102253 | http://purl.uniprot.org/core/pages | "1443-1447"xsd:string |
| http://purl.uniprot.org/citations/22102253 | http://purl.uniprot.org/core/title | "Crystallization and preliminary X-ray data collection of the L27(PATJ)-(L27N,L27C)(Pals1)-L27(MALS) tripartite complex."xsd:string |
| http://purl.uniprot.org/citations/22102253 | http://purl.uniprot.org/core/volume | "67"xsd:string |
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