| http://purl.uniprot.org/citations/22108003 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22108003 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22108003 | http://www.w3.org/2000/01/rdf-schema#comment | "Perhaps the most complex step of macroautophagy is the formation of the double-membrane autophagosome. The majority of the autophagy-related (Atg) proteins are thought to participate in nucleation and expansion of the phagophore, and/or the completion of this compartment. Monitoring this part of the process is difficult, and typically involves electron microscopy analysis; however, unless three-dimensional tomography is performed, even this method cannot be used to easily determine if the phagophore is completely enclosed. Accordingly, a complementary approach is to examine the accessibility of sequestered cargo to exogenously added protease. This type of protease protection analysis has been used to monitor the formation of cytoplasm-to-vacuole targeting (Cvt) vesicles and autophagosomes by examining the protease sensitivity of precursor aminopeptidase I (prApe1). For determining the status of autophagosomes formed during nonselective autophagy, however, prApe1 is not the best marker protein. Here, we describe an alternative method for examining autophagosome completion using GFP-Atg8 as a marker for protease protection."xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.org/dc/terms/identifier | "doi:10.4161/auto.7.12.18424"xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.org/dc/terms/identifier | "doi:10.4161/auto.7.12.18424"xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/author | "Klionsky D.J."xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/author | "Klionsky D.J."xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/author | "Thumm M."xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/author | "Thumm M."xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/author | "Krick R."xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/author | "Krick R."xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/author | "Nair U."xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/author | "Nair U."xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/name | "Autophagy"xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/name | "Autophagy"xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/pages | "1546-1550"xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/pages | "1546-1550"xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/title | "GFP-Atg8 protease protection as a tool to monitor autophagosome biogenesis."xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/title | "GFP-Atg8 protease protection as a tool to monitor autophagosome biogenesis."xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/22108003 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22108003 |
| http://purl.uniprot.org/citations/22108003 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22108003 |