| http://purl.uniprot.org/citations/22152485 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22152485 | http://www.w3.org/2000/01/rdf-schema#comment | "Animal mRNAs are regulated by hundreds of RNA binding proteins (RBPs). The identification of RBP targets is crucial for understanding their function. A recent method, PAR-CLIP, uses photoreactive nucleosides to crosslink RBPs to target RNAs in cells prior to immunoprecipitation. Here, we establish iPAR-CLIP (in vivo PAR-CLIP) to determine, at nucleotide resolution, transcriptome-wide binding sites of GLD-1, a conserved, germline-specific translational repressor in C. elegans. We identified 439 reproducible target mRNAs and demonstrate an excellent dynamic range of target detection by iPAR-CLIP. Upon GLD-1 knockdown, protein but not mRNA expression of the 439 targets was specifically upregulated, demonstrating functionality. Finally, we discovered strongly conserved GLD-1 binding sites near the start codon of target genes. These sites are functional in vitro and likely confer strong repression in vivo. We propose that GLD-1 interacts with the translation machinery near the start codon, a so-far-unknown mode of gene regulation in eukaryotes."xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.molcel.2011.11.009"xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://purl.uniprot.org/core/author | "Kempa S."xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://purl.uniprot.org/core/author | "Mastrobuoni G."xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://purl.uniprot.org/core/author | "Jungkamp A.C."xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://purl.uniprot.org/core/author | "Rajewsky N."xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://purl.uniprot.org/core/author | "Grun D."xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://purl.uniprot.org/core/author | "Mecenas D."xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://purl.uniprot.org/core/author | "Stoeckius M."xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/22152485 | http://purl.uniprot.org/core/name | "Mol Cell"xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://purl.uniprot.org/core/pages | "828-840"xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://purl.uniprot.org/core/title | "In vivo and transcriptome-wide identification of RNA binding protein target sites."xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://purl.uniprot.org/core/volume | "44"xsd:string |
| http://purl.uniprot.org/citations/22152485 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22152485 |
| http://purl.uniprot.org/citations/22152485 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22152485 |
| http://purl.uniprot.org/uniprot/Q17339#attribution-0DBE3638A4AB400EC01653BB30CAAF2F | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22152485 |
| http://purl.uniprot.org/uniprot/Q17339#attribution-FC775A0D3CA5DBDADA225B29D16784E1 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22152485 |
| http://purl.uniprot.org/uniprot/#_Q17339-mappedCitation-22152485 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22152485 |
| http://purl.uniprot.org/uniprot/#_P92186-mappedCitation-22152485 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22152485 |
| http://purl.uniprot.org/uniprot/P92186 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22152485 |
| http://purl.uniprot.org/uniprot/Q17339 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22152485 |