| http://purl.uniprot.org/citations/22166201 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22166201 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22166201 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/22166201 | http://www.w3.org/2000/01/rdf-schema#comment | "Female flowers of hop (Humulus lupulus L.) develop a large number of glandular trichomes called lupulin glands that contain a variety of prenylated compounds such as α- and β-acid (humulone and lupulone, respectively), as well as xanthohumol, a chalcone derivative. These prenylated compounds are biosynthesized by prenyltransferases catalyzing the transfer of dimethylallyl moiety to aromatic substances. In our previous work, we found HlPT-1 a candidate gene for such a prenyltransferase in a cDNA library constructed from lupulin-enriched flower tissues. In this study, we have characterized the enzymatic properties of HlPT-1 using a recombinant protein expressed in baculovirus-infected insect cells. HlPT-1 catalyzed the first transfer of dimethylallyl moiety to phloroglucinol derivatives, phlorisovalerophenone, phlorisobutyrophenone and phlormethylbutanophenone, leading to the formation of humulone and lupulone derivatives. HlPT-1 also recognized naringenin chalcone as a flavonoid substrate to yield xanthohumol, and this broad substrate specificity is a unique character of HlPT-1 that is not seen in other reported flavonoid prenyltransferases, all of which show strict specificity for their aromatic substrates. Moreover, unlike other aromatic substrate prenyltransferases, HlPT-1 revealed an exclusive requirement for Mg(2+) as a divalent cation for its enzymatic activity and also showed exceptionally narrow optimum pH at around pH 7.0."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2011.11.125"xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2011.11.125"xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Uto Y."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Uto Y."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Yazaki K."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Yazaki K."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Sasaki K."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Sasaki K."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Umemoto N."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Umemoto N."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Momma T."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Momma T."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Miyawaki T."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Miyawaki T."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Momose M."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Momose M."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Tsurumaru Y."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/author | "Tsurumaru Y."xsd:string |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22166201 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |