| http://purl.uniprot.org/citations/22171004 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22171004 | http://www.w3.org/2000/01/rdf-schema#comment | "Polynucleotide kinase/phosphatase (PNKP) is a critical mammalian DNA repair enzyme that generates 5'-phosphate and 3'-hydroxyl groups at damaged DNA termini that are required for subsequent processing by DNA ligases and polymerases. The PNKP phosphatase domain recognizes 3'-phosphate termini within DNA nicks, gaps, or at double- or single-strand breaks. Here we present a mechanistic rationale for the recognition of damaged DNA termini by the PNKP phosphatase domain. The crystal structures of PNKP bound to single-stranded DNA substrates reveals a narrow active site cleft that accommodates a single-stranded substrate in a sequence-independent manner. Biochemical studies suggest that the terminal base pairs of double-stranded substrates near the 3'-phosphate are destabilized by PNKP to allow substrate access to the active site. A positively charged surface distinct from the active site specifically facilitates interactions with double-stranded substrates, providing a complex DNA binding surface that enables the recognition of diverse substrates."xsd:string |
| http://purl.uniprot.org/citations/22171004 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1112036108"xsd:string |
| http://purl.uniprot.org/citations/22171004 | http://purl.uniprot.org/core/author | "Coquelle N."xsd:string |
| http://purl.uniprot.org/citations/22171004 | http://purl.uniprot.org/core/author | "Glover J.N."xsd:string |
| http://purl.uniprot.org/citations/22171004 | http://purl.uniprot.org/core/author | "Green R."xsd:string |
| http://purl.uniprot.org/citations/22171004 | http://purl.uniprot.org/core/author | "Havali-Shahriari Z."xsd:string |
| http://purl.uniprot.org/citations/22171004 | http://purl.uniprot.org/core/author | "Bernstein N."xsd:string |
| http://purl.uniprot.org/citations/22171004 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/22171004 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/22171004 | http://purl.uniprot.org/core/pages | "21022-21027"xsd:string |
| http://purl.uniprot.org/citations/22171004 | http://purl.uniprot.org/core/title | "Structural basis for the phosphatase activity of polynucleotide kinase/phosphatase on single- and double-stranded DNA substrates."xsd:string |
| http://purl.uniprot.org/citations/22171004 | http://purl.uniprot.org/core/volume | "108"xsd:string |
| http://purl.uniprot.org/citations/22171004 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22171004 |
| http://purl.uniprot.org/citations/22171004 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22171004 |
| http://purl.uniprot.org/uniprot/#_A0A0J9YTV1-mappedCitation-22171004 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22171004 |
| http://purl.uniprot.org/uniprot/#_A0A0J9YUF9-mappedCitation-22171004 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22171004 |
| http://purl.uniprot.org/uniprot/#_E9Q9A5-mappedCitation-22171004 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22171004 |
| http://purl.uniprot.org/uniprot/#_A0A0J9YUT2-mappedCitation-22171004 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22171004 |
| http://purl.uniprot.org/uniprot/#_A0A0J9YV48-mappedCitation-22171004 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22171004 |
| http://purl.uniprot.org/uniprot/#_A0A0J9YVH5-mappedCitation-22171004 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22171004 |
| http://purl.uniprot.org/uniprot/#_A0A0J9YVJ6-mappedCitation-22171004 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22171004 |
| http://purl.uniprot.org/uniprot/#_A0A140LIZ1-mappedCitation-22171004 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22171004 |
| http://purl.uniprot.org/uniprot/#_A0A140LJ53-mappedCitation-22171004 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22171004 |
| http://purl.uniprot.org/uniprot/#_A0A1S5RA93-mappedCitation-22171004 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22171004 |