| http://purl.uniprot.org/citations/22192732 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22192732 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22192732 | http://www.w3.org/2000/01/rdf-schema#comment | "Disintegrins are low molecular weight peptides isolated from viper venom. These peptides bind to integrin receptors using a conserved binding motif sequence containing an RGD or similar motif. As a consequence, disintegrins can inhibit platelet aggregation and inhibit cell migration, proliferation, and initiate apoptosis in cancer cell lines. Rubistatin is a MVD disintegrin cloned from a Crotalus ruber ruber venom gland. The biological activity of MVD disintegrins is poorly understood. Recombinant rubistatin (r-Rub) was cloned into a pET32b plasmid and expressed in reductase-deficient Escherichia coli. Expression was induced with IPTG and the resulting fusion peptide was affinity purified, followed by thrombin cleavage, and removal of vector coded sequences. r-Rub peptide inhibited ADP-induced platelet aggregation by 54% ± 6.38 in whole blood. We assessed the ability of r-Rub to initiate apoptosis in three human cancer cell lines. Cultures of SK-Mel-28, HeLA, and T24 cells were grown for 24 h with 2.5 μM r-Rub followed by Hoechst staining. Chromatin fragmentation was observed in treated SK-Mel-28, but not in T24 or HeLA cells. A TUNEL assay revealed that 51.55% ± 5.28 of SK-Mel-28 cells were apoptotic after 18 h of treatment with 3.5 μM of r-Rub. Cell migration and proliferation assays were performed in order to further characterize the biological effects of r-Rub on SK-Mel-28 cells. At 3 μM, r-Rub inhibited cell migration by 44.4% ± 0.5, while at 3.5 μM it was able to inhibit cell proliferation by 83% ± 6.0."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.toxicon.2011.12.002"xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.toxicon.2011.12.002"xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Sanchez E.E."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Sanchez E.E."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Bueno R."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Bueno R."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Petro C."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Petro C."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Lucena S.E."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Lucena S.E."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Soto J.G."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Soto J.G."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Carey C.M."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Carey C.M."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Gutierrez D.A."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/author | "Gutierrez D.A."xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/name | "Toxicon"xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/name | "Toxicon"xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/pages | "241-248"xsd:string |
| http://purl.uniprot.org/citations/22192732 | http://purl.uniprot.org/core/pages | "241-248"xsd:string |