| http://purl.uniprot.org/citations/22206988 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22206988 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22206988 | http://www.w3.org/2000/01/rdf-schema#comment | "Activation of fatty acids as acyl-adenylates by fatty acyl-AMP ligases (FAALs) in Mycobacterium tuberculosis is a variant of a classical theme that involves formation of acyl-CoA (coenzyme A) by fatty acyl-CoA ligases (FACLs). Here, we show that FAALs and FACLs possess similar structural fold and substrate specificity determinants, and the key difference is the absence of a unique insertion sequence in FACL13 structure. A systematic analysis shows a conserved hydrophobic anchorage of the insertion motif across several FAALs. Strikingly, mutagenesis of two phenylalanine residues, which are part of the anchorage, to alanine converts FAAL32 to FACL32. This insertion-based in silico analysis suggests the presence of FAAL homologues in several other non-mycobacterial genomes including eukaryotes. The work presented here establishes an elegant mechanism wherein an insertion sequence drives the functional divergence of FAALs from canonical FACLs."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2011.12.031"xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2011.12.031"xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/author | "Gokhale R.S."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/author | "Gokhale R.S."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/author | "Anandhakrishnan M."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/author | "Anandhakrishnan M."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/author | "Goyal A."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/author | "Goyal A."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/author | "Sankaranarayanan R."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/author | "Sankaranarayanan R."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/author | "Verma P."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/author | "Verma P."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/pages | "221-238"xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/pages | "221-238"xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/title | "Molecular basis of the functional divergence of fatty acyl-AMP ligase biosynthetic enzymes of Mycobacterium tuberculosis."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/title | "Molecular basis of the functional divergence of fatty acyl-AMP ligase biosynthetic enzymes of Mycobacterium tuberculosis."xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/volume | "416"xsd:string |
| http://purl.uniprot.org/citations/22206988 | http://purl.uniprot.org/core/volume | "416"xsd:string |