| http://purl.uniprot.org/citations/22214999 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22214999 | http://www.w3.org/2000/01/rdf-schema#comment | "AimsTo mimic, in an animal model of alcoholism, the protective phenotype against alcohol consumption observed in humans carrying a fast alcohol dehydrogenase (ADH1B*2) and an inactive aldehyde dehydrogenase (ALDH2*2).MethodsWe developed a multiple expression cassette adenoviral vector (AdV-ADH/asALDH2) encoding both a fast rat ADH and an antisense RNA against rat ALDH2. A control adenoviral vector (AdV-C) containing intronic non-coding DNA was also developed. These adenoviral vectors were administered intravenously to rats bred as high alcohol-drinkers (University of Chile bibulous) that were previously rendered alcohol dependent by a 75-day period of voluntary 10% ethanol intake.ResultsAnimals administered AdV-ADH/asALDH2 showed a 176% increase in liver ADH activity, whereas liver ALDH2 activity was reduced by 24%, and upon the administration of a dose of ethanol (1 g/kg, i.p.), these showed arterial acetaldehyde levels that were 400% higher than those of animals administered AdV-C. Rats that received the AdV-ADH/asALDH2 vector reduced by 60% their voluntary ethanol intake versus controls.ConclusionThis study provides evidence that the simultaneous increase of liver ADH and a reduction of ALDH activity by gene transfer could constitute a potential therapeutic strategy for the treatment of alcoholism."xsd:string |
| http://purl.uniprot.org/citations/22214999 | http://purl.org/dc/terms/identifier | "doi:10.1093/alcalc/agr161"xsd:string |
| http://purl.uniprot.org/citations/22214999 | http://purl.uniprot.org/core/author | "Quintanilla M.E."xsd:string |
| http://purl.uniprot.org/citations/22214999 | http://purl.uniprot.org/core/author | "Tampier L."xsd:string |
| http://purl.uniprot.org/citations/22214999 | http://purl.uniprot.org/core/author | "Rivera-Meza M."xsd:string |
| http://purl.uniprot.org/citations/22214999 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22214999 | http://purl.uniprot.org/core/name | "Alcohol Alcohol"xsd:string |
| http://purl.uniprot.org/citations/22214999 | http://purl.uniprot.org/core/pages | "102-108"xsd:string |
| http://purl.uniprot.org/citations/22214999 | http://purl.uniprot.org/core/title | "Reduction of ethanol consumption in alcohol-preferring rats by dual expression gene transfer."xsd:string |
| http://purl.uniprot.org/citations/22214999 | http://purl.uniprot.org/core/volume | "47"xsd:string |
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