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http://purl.uniprot.org/citations/22230500http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22230500http://www.w3.org/2000/01/rdf-schema#comment"

Aim

To investigate the effects and possible molecular mechanisms of inhibiting FOXM1 expression on SKOV3 cells by lentiviral vector targeting FOXM1 shRNA.

Methods

SKOV3 cells were infected by lentiviral vector targeting FOXM1 shRNA with a multiplicity of infection (MOI) of 20, then growth curve of SKOV3 cells was determined by MTT assay, cell cycle was analysed by flow cytometry(FCM), and the expression of mRNA and protein of FOXM1, Cyclin D1, PLK1 by Real time PCR and Western blot.

Results

Lentiviral vector targeting FOXM1 shRNA with a multiplicity of infection (MOI) of 20 could significantly inhibit the growth of SKOV3 cells. After infected by lentiviral vector targeting FOXM1 shRNA, the G(0);/G(1); phase cells increased and the S-phase cells decreased, and the expression of mRNA and protein of FOXM1, Cyclin D1, PLK1 of SKOV3 cells were significantly down-regulated.

Conclusion

Inhibiting FOXM1 expression has a significantly effect of inhibiting proliferation on SKOV3 cells. Blocking SKOV3 cells in the G(0);/G(1); phase by down-regulating the expression of Cyclin D1, PLK1 protein may be its mechanism."xsd:string
http://purl.uniprot.org/citations/22230500http://purl.uniprot.org/core/author"Chen G.Q."xsd:string
http://purl.uniprot.org/citations/22230500http://purl.uniprot.org/core/author"Luo Y."xsd:string
http://purl.uniprot.org/citations/22230500http://purl.uniprot.org/core/author"Zhang H."xsd:string
http://purl.uniprot.org/citations/22230500http://purl.uniprot.org/core/author"Qin X.F."xsd:string
http://purl.uniprot.org/citations/22230500http://purl.uniprot.org/core/author"Qi H.B."xsd:string
http://purl.uniprot.org/citations/22230500http://purl.uniprot.org/core/author"Yao Z.W."xsd:string
http://purl.uniprot.org/citations/22230500http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22230500http://purl.uniprot.org/core/name"Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/22230500http://purl.uniprot.org/core/pages"29-32"xsd:string
http://purl.uniprot.org/citations/22230500http://purl.uniprot.org/core/title"[Effects and molecular mechanisms of inhibiting FOXM1 expression on ovarian cancer cell line SKOV3 by Lentiviral vector]."xsd:string
http://purl.uniprot.org/citations/22230500http://purl.uniprot.org/core/volume"28"xsd:string
http://purl.uniprot.org/citations/22230500http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/22230500
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