| http://purl.uniprot.org/citations/22247292 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22247292 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22247292 | http://www.w3.org/2000/01/rdf-schema#comment | "The dicistrovirus intergenic region internal ribosome entry site (IRES) utilizes a unique mechanism, involving P-site tRNA mimicry, to directly assemble 80S ribosomes and initiate translation at a specific non-AUG codon in the ribosomal A site. A subgroup of dicistrovirus genomes contains an additional stem-loop 5'-adjacent to the IRES and a short open reading frame (ORFx) that overlaps the viral structural polyprotein ORF (ORF2) in the +1 reading frame. Using mass spectrometry and extensive mutagenesis, we show that, besides directing ORF2 translation, the Israeli acute paralysis dicistrovirus IRES also directs ORFx translation. The latter is mediated by a UG base pair adjacent to the P-site tRNA-mimicking domain. An ORFx peptide was detected in virus-infected honey bees by multiple reaction monitoring mass spectrometry. Finally, the 5' stem-loop increases IRES activity and may couple translation of the two major ORFs of the virus. This study reveals a novel viral strategy in which a tRNA-like IRES directs precise, initiator Met-tRNA-independent translation of two overlapping ORFs."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1111303109"xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1111303109"xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Atkins J.F."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Atkins J.F."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Chan M.M."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Chan M.M."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Foster L.J."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Foster L.J."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Jan E."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Jan E."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Ren Q."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Ren Q."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Firth A.E."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Firth A.E."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Gouw J.W."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Gouw J.W."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Guarna M.M."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Guarna M.M."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Wang Q.S."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/author | "Wang Q.S."xsd:string |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22247292 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |