| http://purl.uniprot.org/citations/22249180 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22249180 | http://www.w3.org/2000/01/rdf-schema#comment | "Juvenile hormone (JH) has been implicated in many developmental processes in holometabolous insects, but its mechanism of signaling remains controversial. We previously found that in Drosophila Schneider 2 cells, the nuclear receptor FTZ-F1 is required for activation of the E75A gene by JH. Here, we utilized insect two-hybrid assays to show that FTZ-F1 interacts with two JH receptor candidates, the bHLH-PAS paralogs MET and GCE, in a JH-dependent manner. These interactions are severely reduced when helix 12 of the FTZ-F1 activation function 2 (AF2) is removed, implicating AF2 as an interacting site. Through homology modeling, we found that MET and GCE possess a C-terminal α-helix featuring a conserved motif LIXXL that represents a novel nuclear receptor (NR) box. Docking simulations supported by two-hybrid experiments revealed that FTZ-F1·MET and FTZ-F1·GCE heterodimer formation involves a typical NR box-AF2 interaction but does not require the canonical charge clamp residues of FTZ-F1 and relies primarily on hydrophobic contacts, including a unique interaction with helix 4. Moreover, we identified paralog-specific features, including a secondary interaction site found only in MET. Our findings suggest that a novel NR box enables MET and GCE to interact JH-dependently with the AF2 of FTZ-F1."xsd:string |
| http://purl.uniprot.org/citations/22249180 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.327254"xsd:string |
| http://purl.uniprot.org/citations/22249180 | http://purl.uniprot.org/core/author | "Dubrovsky E.B."xsd:string |
| http://purl.uniprot.org/citations/22249180 | http://purl.uniprot.org/core/author | "Bernardo T.J."xsd:string |
| http://purl.uniprot.org/citations/22249180 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22249180 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/22249180 | http://purl.uniprot.org/core/pages | "7821-7833"xsd:string |
| http://purl.uniprot.org/citations/22249180 | http://purl.uniprot.org/core/title | "The Drosophila juvenile hormone receptor candidates methoprene-tolerant (MET) and germ cell-expressed (GCE) utilize a conserved LIXXL motif to bind the FTZ-F1 nuclear receptor."xsd:string |
| http://purl.uniprot.org/citations/22249180 | http://purl.uniprot.org/core/volume | "287"xsd:string |
| http://purl.uniprot.org/citations/22249180 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22249180 |
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