| http://purl.uniprot.org/citations/22249291 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22249291 | http://www.w3.org/2000/01/rdf-schema#comment | "Accurate DNA replication requires proper regulation of replication licensing, which entails loading MCM-2-7 onto replication origins. In this paper, we provide the first comprehensive view of replication licensing in vivo, using video microscopy of Caenorhabditis elegans embryos. As expected, MCM-2-7 loading in late M phase depended on the prereplicative complex (pre-RC) proteins: origin recognition complex (ORC), CDC-6, and CDT-1. However, many features we observed have not been described before: GFP-ORC-1 bound chromatin independently of ORC-2-5, and CDC-6 bound chromatin independently of ORC, whereas CDT-1 and MCM-2-7 DNA binding was interdependent. MCM-3 chromatin loading was irreversible, but CDC-6 and ORC turned over rapidly, consistent with ORC/CDC-6 loading multiple MCM-2-7 complexes. MCM-2-7 chromatin loading further reduced ORC and CDC-6 DNA binding. This dynamic behavior creates a feedback loop allowing ORC/CDC-6 to repeatedly load MCM-2-7 and distribute licensed origins along chromosomal DNA. During S phase, ORC and CDC-6 were excluded from nuclei, and DNA was overreplicated in export-defective cells. Thus, nucleocytoplasmic compartmentalization of licensing factors ensures that DNA replication occurs only once."xsd:string |
| http://purl.uniprot.org/citations/22249291 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.201110080"xsd:string |
| http://purl.uniprot.org/citations/22249291 | http://purl.uniprot.org/core/author | "Craig A."xsd:string |
| http://purl.uniprot.org/citations/22249291 | http://purl.uniprot.org/core/author | "Gartner A."xsd:string |
| http://purl.uniprot.org/citations/22249291 | http://purl.uniprot.org/core/author | "Blow J.J."xsd:string |
| http://purl.uniprot.org/citations/22249291 | http://purl.uniprot.org/core/author | "Sonneville R."xsd:string |
| http://purl.uniprot.org/citations/22249291 | http://purl.uniprot.org/core/author | "Querenet M."xsd:string |
| http://purl.uniprot.org/citations/22249291 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22249291 | http://purl.uniprot.org/core/name | "J Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/22249291 | http://purl.uniprot.org/core/pages | "233-246"xsd:string |
| http://purl.uniprot.org/citations/22249291 | http://purl.uniprot.org/core/title | "The dynamics of replication licensing in live Caenorhabditis elegans embryos."xsd:string |
| http://purl.uniprot.org/citations/22249291 | http://purl.uniprot.org/core/volume | "196"xsd:string |
| http://purl.uniprot.org/citations/22249291 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22249291 |
| http://purl.uniprot.org/citations/22249291 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22249291 |
| http://purl.uniprot.org/uniprot/Q9XXI9#attribution-004876C0AA8CF65E6FD2A436996ADE0B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22249291 |
| http://purl.uniprot.org/uniprot/Q9XXI9#attribution-86AB710DF6162E758CAFA0EC82ADE61B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22249291 |
| http://purl.uniprot.org/uniprot/O16297#attribution-004876C0AA8CF65E6FD2A436996ADE0B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22249291 |
| http://purl.uniprot.org/uniprot/O16297#attribution-3FCF48AC71303F449429687C6DDD042C | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22249291 |
| http://purl.uniprot.org/uniprot/Q9XVR7#attribution-004876C0AA8CF65E6FD2A436996ADE0B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22249291 |
| http://purl.uniprot.org/uniprot/Q9XVR7#attribution-86AB710DF6162E758CAFA0EC82ADE61B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22249291 |
| http://purl.uniprot.org/uniprot/Q7K7J6#attribution-004876C0AA8CF65E6FD2A436996ADE0B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22249291 |
| http://purl.uniprot.org/uniprot/Q7K7J6#attribution-86AB710DF6162E758CAFA0EC82ADE61B | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22249291 |
| http://purl.uniprot.org/uniprot/#_A0A1X7RCW1-mappedCitation-22249291 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22249291 |
| http://purl.uniprot.org/uniprot/#_D3YT51-mappedCitation-22249291 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22249291 |