| http://purl.uniprot.org/citations/22257160 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22257160 | http://www.w3.org/2000/01/rdf-schema#comment | "The Caenorhabditis elegans Werner syndrome protein, WRN-1, a member of the RecQ helicase family, has a 3'-5' DNA helicase activity. Worms with defective wrn-1 exhibit premature aging phenotypes and an increased level of genome instability. In response to DNA damage, WRN-1 participates in the initial stages of checkpoint activation in concert with C. elegans replication protein A (RPA-1). WRN-1 helicase is stimulated by RPA-1 on long DNA duplex substrates. However, the mechanism by which RPA-1 stimulates DNA unwinding and the function of the WRN-1-RPA-1 interaction are not clearly understood. We have found that WRN-1 physically interacts with two RPA-1 subunits, CeRPA73 and CeRPA32; however, full-length WRN-1 helicase activity is stimulated by only the CeRPA73 subunit, while the WRN-1(162-1056) fragment that harbors the helicase activity requires both the CeRPA73 and CeRPA32 subunits for the stimulation. We also found that the CeRPA73(1-464) fragment can stimulate WRN-1 helicase activity and that residues 335-464 of CeRPA73 are important for physical interaction with WRN-1. Because CeRPA73 and the CeRPA73(1-464) fragment are able to bind single-stranded DNA (ssDNA), the stimulation of WRN-1 helicase by RPA-1 is most likely due to the ssDNA binding activity of CeRPA73 and the direct interaction of WRN-1 and CeRPA73."xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi200791p"xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/author | "Kim E."xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/author | "Kim D.H."xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/author | "Lee S.J."xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/author | "Park S."xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/author | "Koo H.S."xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/author | "Seo Y.S."xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/author | "Ahn B."xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/author | "Hyun M."xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/pages | "1336-1345"xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/title | "Physical and functional interactions of Caenorhabditis elegans WRN-1 helicase with RPA-1."xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://purl.uniprot.org/core/volume | "51"xsd:string |
| http://purl.uniprot.org/citations/22257160 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22257160 |
| http://purl.uniprot.org/citations/22257160 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22257160 |
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| http://purl.uniprot.org/uniprot/#_Q95Y97-mappedCitation-22257160 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22257160 |