| http://purl.uniprot.org/citations/22261724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22261724 | http://www.w3.org/2000/01/rdf-schema#comment | "Asparagine-linked glycosylation is a common post-translational modification of proteins; in addition to participating in key macromolecular interactions, N-glycans contribute to protein folding, trafficking, and stability. Despite their importance, few N-glycosites have been experimentally mapped in the Saccharomyces cerevisiae proteome. Factors including glycan heterogeneity, low abundance, and low occupancy can complicate site mapping. Here, we report a novel mass spectrometry-based strategy for detection of N-glycosites in the yeast proteome. Our method imparts N-glycopeptide mass envelopes with a pattern that is computationally distinguishable from background ions. Isotopic recoding is achieved via metabolic incorporation of a defined mixture of N-acetylglucosamine isotopologs into N-glycans. Peptides bearing the recoded envelopes are specifically targeted for fragmentation, facilitating high confidence site mapping. This strategy requires no chemical modification of the N-glycans or stringent sample enrichment. Further, enzymatically simplified N-glycans are preserved on peptides. Using this approach, we identify 133 N-glycosites spanning 58 proteins, nearly doubling the number of experimentally observed N-glycosites in the yeast proteome."xsd:string |
| http://purl.uniprot.org/citations/22261724 | http://purl.org/dc/terms/identifier | "doi:10.1074/mcp.m111.015339"xsd:string |
| http://purl.uniprot.org/citations/22261724 | http://purl.uniprot.org/core/author | "Breidenbach M.A."xsd:string |
| http://purl.uniprot.org/citations/22261724 | http://purl.uniprot.org/core/author | "Bertozzi C.R."xsd:string |
| http://purl.uniprot.org/citations/22261724 | http://purl.uniprot.org/core/author | "Palaniappan K.K."xsd:string |
| http://purl.uniprot.org/citations/22261724 | http://purl.uniprot.org/core/author | "Pitcher A.A."xsd:string |
| http://purl.uniprot.org/citations/22261724 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22261724 | http://purl.uniprot.org/core/name | "Mol Cell Proteomics"xsd:string |
| http://purl.uniprot.org/citations/22261724 | http://purl.uniprot.org/core/pages | "M111.015339"xsd:string |
| http://purl.uniprot.org/citations/22261724 | http://purl.uniprot.org/core/title | "Mapping yeast N-glycosites with isotopically recoded glycans."xsd:string |
| http://purl.uniprot.org/citations/22261724 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/22261724 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22261724 |
| http://purl.uniprot.org/citations/22261724 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22261724 |
| http://purl.uniprot.org/uniprot/#_P54003-mappedCitation-22261724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22261724 |
| http://purl.uniprot.org/uniprot/#_P38993-mappedCitation-22261724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22261724 |
| http://purl.uniprot.org/uniprot/#_P39007-mappedCitation-22261724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22261724 |
| http://purl.uniprot.org/uniprot/#_P39105-mappedCitation-22261724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22261724 |
| http://purl.uniprot.org/uniprot/#_P40006-mappedCitation-22261724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22261724 |
| http://purl.uniprot.org/uniprot/#_P42835-mappedCitation-22261724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22261724 |
| http://purl.uniprot.org/uniprot/#_P41543-mappedCitation-22261724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22261724 |
| http://purl.uniprot.org/uniprot/#_P00635-mappedCitation-22261724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22261724 |
| http://purl.uniprot.org/uniprot/#_P00729-mappedCitation-22261724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22261724 |
| http://purl.uniprot.org/uniprot/#_P24031-mappedCitation-22261724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22261724 |
| http://purl.uniprot.org/uniprot/#_Q06689-mappedCitation-22261724 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22261724 |