| http://purl.uniprot.org/citations/22264607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22264607 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22264607 | http://www.w3.org/2000/01/rdf-schema#comment | "Myosin IIIA (MYO3A) targets actin protrusion tips using a motility mechanism dependent on both motor and tail actin-binding activity [1]. We show that myosin IIIB (MYO3B) lacks tail actin-binding activity and is unable to target COS7 cell filopodia tips, yet is somehow able to target stereocilia tips. Strikingly, when MYO3B is coexpressed with espin-1 (ESPN1), a MYO3A cargo protein endogenously expressed in stereocilia [2], MYO3B targets and carries ESPN1 to COS7 filopodia tips. We show that this tip localization is lost when we remove the ESPN1 C terminus actin-binding site. We also demonstrate that, like MYO3A [2], MYO3B can elongate filopodia by transporting ESPN1 to the polymerizing end of actin filaments. The mutual dependence of MYO3B and ESPN1 for tip localization reveals a novel mechanism for the cell to regulate myosin tip localization via a reciprocal relationship with cargo that directly participates in actin binding for motility. Our results are consistent with a novel form of motility for class III myosins that requires both motor and tail domain actin-binding activity and show that the actin-binding tail can be replaced by actin-binding cargo. This study also provides a framework to better understand the late-onset hearing loss phenotype in patients with MYO3A mutations."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cub.2011.12.053"xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cub.2011.12.053"xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Manor U."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Manor U."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Grati M."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Grati M."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Kachar B."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Kachar B."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Dose A.C."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Dose A.C."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Salles F.T."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Salles F.T."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Merritt R.C."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Merritt R.C."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Yengo C.M."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Yengo C.M."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Quintero O.A."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Quintero O.A."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Unrath W.C."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/author | "Unrath W.C."xsd:string |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22264607 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |