| http://purl.uniprot.org/citations/22281743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22281743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22281743 | http://www.w3.org/2000/01/rdf-schema#comment | "Octahaem cytochrome c nitrite reductase from Thioalkalivibrio nitratireducens (TvNiR), like the previously characterized pentahaem nitrite reductases (NrfAs), catalyzes the six-electron reductions of nitrite to ammonia and of sulfite to sulfide. The active site of both TvNiR and NrfAs is formed by the lysine-coordinated haem and His, Tyr and Arg residues. The distinguishing structural feature of TvNiR is the presence of a covalent bond between the CE2 atom of the catalytic Tyr303 and the S atom of Cys305, which might be responsible for the higher nitrite reductase activity of TvNiR compared with NrfAs. In the present study, a new modified form of the enzyme (TvNiRb) that contains an additional covalent bond between Tyr303 CE1 and Gln360 CG is reported. Structures of TvNiRb in complexes with phosphate (1.45 Å resolution) and sulfite (1.8 Å resolution), the structure of TvNiR in a complex with nitrite (1.83 Å resolution) and several additional structures were determined. The formation of the second covalent bond by Tyr303 leads to a decrease in both the nitrite and sulfite reductase activities of the enzyme. Tyr303 is located at the exit from the putative proton-transport channel to the active site, which is absent in NrfAs. This is an additional argument in favour of the involvement of Tyr303 as a proton donor in catalysis. The changes in the activity of cytochrome c nitrite reductases owing to the formation of Tyr-Cys and Tyr-Gln bonds may be associated with changes in the pK(a) value of the catalytic tyrosine."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.org/dc/terms/identifier | "doi:10.1107/s0907444911052632"xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.org/dc/terms/identifier | "doi:10.1107/s0907444911052632"xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Polyakov K.M."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Polyakov K.M."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Popov V.O."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Popov V.O."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Tikhonova T.V."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Tikhonova T.V."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Boyko K.M."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Boyko K.M."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Dorovatovskii P.V."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Dorovatovskii P.V."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Safonova T.N."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Safonova T.N."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Tikhonov A.V."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Tikhonov A.V."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Trofimov A.A."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/author | "Trofimov A.A."xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/name | "Acta Crystallogr. D"xsd:string |
| http://purl.uniprot.org/citations/22281743 | http://purl.uniprot.org/core/name | "Acta Crystallogr. D"xsd:string |