| http://purl.uniprot.org/citations/22302984 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22302984 | http://www.w3.org/2000/01/rdf-schema#comment | "Intracellular bacteria have been shown to cause autophagy, which impacts infectious outcomes, whereas extracellular bacteria have not been reported to activate autophagy. Here, we demonstrate that Pseudomonas aeruginosa, a Gram-negative extracellular bacterium, activates autophagy with considerably increased LC3 punctation in both an alveolar macrophage cell line (MH-S) and primary alveolar macrophages. Using the LC3 Gly120 mutant, we successfully demonstrated a hallmark of autophagy, conjugation of LC3 to phosphatidylethanolamine (PE). The accumulation of typical autophagosomes with double membranes was identified morphologically by transmission electron microscopy (TEM). Furthermore, the increase of PE-conjugated LC3 was indeed induced by infection rather than inhibition of lysosome degradation. P. aeruginosa induced autophagy through the classical beclin-1-Atg7-Atg5 pathway as determined by specific siRNA analysis. Rapamycin and IFN-γ (autophagy inducers) augmented bacterial clearance, whereas beclin-1 and Atg5 knockdown reduced intracellular bacteria. Thus, P. aeruginosa-induced autophagy represents a host protective mechanism, providing new insight into the pathogenesis of this infection."xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.org/dc/terms/identifier | "doi:10.1242/jcs.094573"xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/author | "Gao H."xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/author | "Huang C."xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/author | "Wu M."xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/author | "Zhang B."xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/author | "Yin Q."xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/author | "Yuan K."xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/author | "Fox J."xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/author | "Carlson E."xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/author | "Laturnus D."xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/name | "J Cell Sci"xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/pages | "507-515"xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/title | "Autophagy plays an essential role in the clearance of Pseudomonas aeruginosa by alveolar macrophages."xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://purl.uniprot.org/core/volume | "125"xsd:string |
| http://purl.uniprot.org/citations/22302984 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22302984 |
| http://purl.uniprot.org/citations/22302984 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22302984 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MQN4-mappedCitation-22302984 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22302984 |
| http://purl.uniprot.org/uniprot/#_E9QAM0-mappedCitation-22302984 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22302984 |
| http://purl.uniprot.org/uniprot/#_A0A385JBX3-mappedCitation-22302984 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22302984 |
| http://purl.uniprot.org/uniprot/#_F7C090-mappedCitation-22302984 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22302984 |
| http://purl.uniprot.org/uniprot/#_F7CVL9-mappedCitation-22302984 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22302984 |
| http://purl.uniprot.org/uniprot/#_E9PX06-mappedCitation-22302984 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22302984 |