| http://purl.uniprot.org/citations/2230956 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2230956 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2230956 | http://www.w3.org/2000/01/rdf-schema#comment | "A fully encoding cDNA for the high-molecular-weight rat neurofilament protein (NF-H) has been isolated from a lambda gt11 library, sequenced and subcloned into eukaryotic expression vectors. Sequence analysis shows that rat NF-H has an overall homology of 72 and 88% with human and mouse NF-H, respectively. The head and rod domains are almost entirely identical, and the divergences are due to differences in the long C-terminal extensions of the molecule. The consensus phosphorylation sequence for neurofilaments Lys-Ser-Pro (KSP) is present 52 times. The predicted molecular mass of the protein is 115 kDa, 42% lower than that observed by SDS-PAGE. Upon transfection into vimentin-containing fibroblasts, such as L tk-, L929, and 3T6 cells, NF-H is seen distributed with vimentin by light and electron microscopic examinations indicating that copolymers of NF-H and vimentin are formed in these cells. Only a negligible proportion of the cells is positive when stained with a number of antibodies directed against phosphorylated NF-H epitopes. This is in contrast with the middle molecular weight NF protein (NF-M) transfected into L tk- and L929 cells, which can readily be detected by antibodies against phosphorylated neurofilament epitopes. The mobilities of the transfected protein on 1- and 2-dimensional gels confirm that NF-H is predominantly in a nonphosphorylated form. These results indicate that phosphorylation of NF-H, but not NF-M, on the KSP sequence is due to protein kinases, which are not present in fibroblasts and are presumably NF-H specific. The stable NF-H-expressing cell lines can therefore be used to study these putative neurofilament kinases in vitro and in vivo."xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.10-11-03714.1990"xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.org/dc/terms/identifier | "doi:10.1523/jneurosci.10-11-03714.1990"xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/author | "Liem R.K."xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/author | "Liem R.K."xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/author | "Chin S.S."xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/author | "Chin S.S."xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/name | "J. Neurosci."xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/name | "J. Neurosci."xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/pages | "3714-3726"xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/pages | "3714-3726"xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/title | "Transfected rat high-molecular-weight neurofilament (NF-H) coassembles with vimentin in a predominantly nonphosphorylated form."xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/title | "Transfected rat high-molecular-weight neurofilament (NF-H) coassembles with vimentin in a predominantly nonphosphorylated form."xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/2230956 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2230956 |
| http://purl.uniprot.org/citations/2230956 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/2230956 |
| http://purl.uniprot.org/citations/2230956 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2230956 |
| http://purl.uniprot.org/citations/2230956 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/2230956 |
| http://purl.uniprot.org/uniprot/P16884 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/2230956 |
| http://purl.uniprot.org/embl-cds/AAB87068.1 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/2230956 |