| http://purl.uniprot.org/citations/22350815 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22350815 | http://www.w3.org/2000/01/rdf-schema#comment | "ZNF403, also known as GGNBP2 (gametogenetin binding protein 2), is a highly conserved gene implicated in spermatogenesis. However, the exact biological function of ZNF403 is not clear. In this study, we identified the role of ZNF403 in cell proliferation and cell-cycle regulation by utilizing short hairpin RNA (shRNA)-mediated knockdown. ZNF403-specific shRNA expressing helper-dependent adenoviral vector (HD-Ad-ZNF403-shRNA) was constructed and transduced human cell lines. ZNF403 mRNA and protein expression levels were inhibited as evidenced by real-time PCR and western blot analyses. Noticeably, we found that knockdown of ZNF403 expression suppressed cell proliferation compared to the non-target shRNA and vector controls. Furthermore, cell-cycle analysis demonstrated that downregulation of ZNF403 promoted G2/M cell-cycle arrest in a dose-dependent manner. Moreover, human cell-cycle real-time PCR array revealed that ZNF403 knockdown influenced the expression profile of genes in cell-cycle regulation. Among these genes, western blot analysis confirmed the protein up-regulation of p21 and down-regulation of MCM2 in response to the ZNF403 knockdown. Additionally, knockdown of ZNF403 also showed an anti-carcinogenetic effect on anchorage-independent growth by colony formation assay and tumor cell migration by wound-healing assay with human laryngeal cancer cell line Hep-2 cells. Altogether, our findings suggest an essential role of ZNF403 in cell proliferation and provide a new insight into the function of ZNF403 in regulating the G2/M cell-cycle transition."xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11010-012-1262-6"xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/author | "Cao H."xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/author | "Chen Z."xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/author | "Duan R."xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/author | "Hu J."xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/author | "Hou D."xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/author | "Wu J."xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/author | "Wen X.Y."xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/author | "Guan R."xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/author | "Lam S."xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/name | "Mol Cell Biochem"xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/pages | "211-222"xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/title | "Knockdown of ZNF403 inhibits cell proliferation and induces G2/M arrest by modulating cell-cycle mediators."xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://purl.uniprot.org/core/volume | "365"xsd:string |
| http://purl.uniprot.org/citations/22350815 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22350815 |
| http://purl.uniprot.org/citations/22350815 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22350815 |
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| http://purl.uniprot.org/uniprot/#_Q9H3C7-mappedCitation-22350815 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22350815 |