| http://purl.uniprot.org/citations/22384376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22384376 | http://www.w3.org/2000/01/rdf-schema#comment | "The multifunctional factors Imp-α and Imp-β are involved in nuclear protein import, mitotic spindle dynamics, and nuclear membrane formation. Furthermore, each of the three members of the Imp-α family exerts distinct tasks during development. In Drosophila melanogaster, the imp-α2 gene is critical during oogenesis for ring canal assembly; specific mutations, which allow oogenesis to proceed normally, were found to block early embryonic mitosis. Here, we show that imp-α2 and imp-β genetically interact during early embryonic development, and we characterize the pattern of defects affecting mitosis in embryos laid by heterozygous imp-α2(D14) and imp-β(KetRE34) females. Embryonic development is arrested in these embryos but is unaffected in combinations between imp-β(KetRE34) and null mutations in imp-α1 or imp-α3. Furthermore, the imp-α2(D14)/imp-β(KetRE34) interaction could only be rescued by an imp-α2 transgene, albeit not imp-α1 or imp-α3, showing the exclusive imp-α2 function with imp-β. Use of transgenes carrying modifications in the major Imp-α2 domains showed the critical requirement of the nuclear localization signal binding (NLSB) site in this process. In the mutant embryos, we found metaphase-arrested mitoses made of enlarged spindles, suggesting an unrestrained activity of factors promoting spindle assembly. In accordance with this, we found that Imp-β(KetRE34) and Imp-β(KetD) bind a high level of RanGTP/GDP, and a deletion decreasing RanGTP level suppresses the imp-β(KetRE34) phenotype. These data suggest that a fine balance among Imp-α2, Imp-β, RanGTP, and the NLS cargos is critical for mitotic progression during early embryonic development."xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.org/dc/terms/identifier | "doi:10.1534/g3.111.001073"xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/author | "Gorjanacz M."xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/author | "Kiss I."xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/author | "Mechler B.M."xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/author | "Viragh E."xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/author | "Torok I."xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/author | "Szlanka T."xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/author | "Kallakuri S."xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/author | "Eichhorn T."xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/name | "G3 (Bethesda)"xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/pages | "1-14"xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/title | "Specific Cooperation Between Imp-alpha2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions."xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://purl.uniprot.org/core/volume | "2"xsd:string |
| http://purl.uniprot.org/citations/22384376 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22384376 |
| http://purl.uniprot.org/citations/22384376 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22384376 |
| http://purl.uniprot.org/uniprot/#_A4V4A5-mappedCitation-22384376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22384376 |
| http://purl.uniprot.org/uniprot/#_O18388-mappedCitation-22384376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22384376 |
| http://purl.uniprot.org/uniprot/#_P52295-mappedCitation-22384376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22384376 |
| http://purl.uniprot.org/uniprot/#_M9PBD9-mappedCitation-22384376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22384376 |
| http://purl.uniprot.org/uniprot/#_M9NE89-mappedCitation-22384376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22384376 |
| http://purl.uniprot.org/uniprot/#_Q24368-mappedCitation-22384376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22384376 |
| http://purl.uniprot.org/uniprot/#_Q24478-mappedCitation-22384376 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22384376 |