| http://purl.uniprot.org/citations/22385962 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22385962 | http://www.w3.org/2000/01/rdf-schema#comment | "Lamellipodia are sheet-like, leading edge protrusions in firmly adherent cells that contain Arp2/3-generated dendritic actin networks. Although lamellipodia are widely believed to be critical for directional cell motility, this notion has not been rigorously tested. Using fibroblasts derived from Ink4a/Arf-deficient mice, we generated a stable line depleted of Arp2/3 complex that lacks lamellipodia. This line shows defective random cell motility and relies on a filopodia-based protrusion system. Utilizing a microfluidic gradient generation system, we tested the role of Arp2/3 complex and lamellipodia in directional cell migration. Surprisingly, Arp2/3-depleted cells respond normally to shallow gradients of PDGF, indicating that lamellipodia are not required for fibroblast chemotaxis. Conversely, these cells cannot respond to a surface-bound gradient of extracellular matrix (haptotaxis). Consistent with this finding, cells depleted of Arp2/3 fail to globally align focal adhesions, suggesting that one principle function of lamellipodia is to organize cell-matrix adhesions in a spatially coherent manner."xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.cell.2011.12.034"xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/author | "Gomez S.M."xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/author | "Wu C."xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/author | "Griffith J.D."xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/author | "Bear J.E."xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/author | "Sharpless N.E."xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/author | "Berginski M.E."xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/author | "Asokan S.B."xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/author | "Haynes E.M."xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/name | "Cell"xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/pages | "973-987"xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/title | "Arp2/3 is critical for lamellipodia and response to extracellular matrix cues but is dispensable for chemotaxis."xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://purl.uniprot.org/core/volume | "148"xsd:string |
| http://purl.uniprot.org/citations/22385962 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22385962 |
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