| http://purl.uniprot.org/citations/22393000 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22393000 | http://www.w3.org/2000/01/rdf-schema#comment | "During vertebrate cytokinesis it is thought that contractile ring constriction is driven by nonmuscle myosin II (NM II) translocation of antiparallel actin filaments. Here we report in situ, in vitro, and in vivo observations that challenge this hypothesis. Graded knockdown of NM II in cultured COS-7 cells reveals that the amount of NM II limits ring constriction. Restoration of the constriction rate with motor-impaired NM II mutants shows that the ability of NM II to translocate actin is not required for cytokinesis. Blebbistatin inhibition of cytokinesis indicates the importance of myosin strongly binding to actin and exerting tension during cytokinesis. This role is substantiated by transient kinetic experiments showing that the load-dependent mechanochemical properties of mutant NM II support efficient tension maintenance despite the inability to translocate actin. Under loaded conditions, mutant NM II exhibits a prolonged actin attachment in which a single mechanoenzymatic cycle spans most of the time of cytokinesis. This prolonged attachment promotes simultaneous binding of NM II heads to actin, thereby increasing tension and resisting expansion of the ring. The detachment of mutant NM II heads from actin is enhanced by assisting loads, which prevent mutant NM II from hampering furrow ingression during cytokinesis. In the 3D context of mouse hearts, mutant NM II-B R709C that cannot translocate actin filaments can rescue multinucleation in NM II-B ablated cardiomyocytes. We propose that the major roles of NM II in vertebrate cell cytokinesis are to bind and cross-link actin filaments and to exert tension on actin during contractile ring constriction."xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.1116268109"xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://purl.uniprot.org/core/author | "Ma X."xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://purl.uniprot.org/core/author | "Zhang Y."xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://purl.uniprot.org/core/author | "Wang A."xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://purl.uniprot.org/core/author | "Sellers J.R."xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://purl.uniprot.org/core/author | "Kovacs M."xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://purl.uniprot.org/core/author | "Adelstein R.S."xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://purl.uniprot.org/core/author | "Conti M.A."xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22393000 | http://purl.uniprot.org/core/name | "Proc Natl Acad Sci U S A"xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://purl.uniprot.org/core/pages | "4509-4514"xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://purl.uniprot.org/core/title | "Nonmuscle myosin II exerts tension but does not translocate actin in vertebrate cytokinesis."xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://purl.uniprot.org/core/volume | "109"xsd:string |
| http://purl.uniprot.org/citations/22393000 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22393000 |
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| http://purl.uniprot.org/uniprot/#_Q14B05-mappedCitation-22393000 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22393000 |
| http://purl.uniprot.org/uniprot/#_Q8BLI1-mappedCitation-22393000 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22393000 |
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| http://purl.uniprot.org/uniprot/#_Q99JJ3-mappedCitation-22393000 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22393000 |