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http://purl.uniprot.org/citations/22394632http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22394632http://www.w3.org/2000/01/rdf-schema#comment"

Aim

To construct pCEP4/hIL-17B recombinant expression vector and express it stably in eukaryotic cells and investigate the biological activity in vitro.

Methods

The CDS region of human IL-17B gene was cloned by RT-PCR. After identification by sequencing, the hIL-17B gene was inserted into expression vector of pCEP4 to construct the recombinant vector pCEP4/hIL-17B, then transfected into 293T cells. The transgenic 293T cell line stably expressing rhIL-17B protein was selected in the presence of Hygromycin B. After FCS-free cultivation and sub-cloning, The IL-17B/mFc gene and protein expression was confirmed by RT-PCR, ELISA and Western blot analysis. To investigate the ability of combination with IL-17B receptor on human leukemic monocytic cell line, THP-1, by Flow cytometrical analysis (FACS) and of stimulation to secret cytokines in vitro.

Results

The recombinant pCEP4/hIL-17B and its transgenic 293T cells stably expressing rhIL-17B protein were obtained successfully. FACS analysis showed its high affinity with its receptor and it can stimulated THP-1 cell line to excrete IL-1β and TNF-α in vitro and consistently caused a dose-dependent influx of neutrophil into the peritoneal cavity by intraperitoneal injection in vivo.

Conclusion

The obtainment of transgenic 293T cell line stably expressing rhIL-17B protein paved the way for further study on biological functions of hIL-17B."xsd:string
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/author"Zhang X.G."xsd:string
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/author"Zhu X.F."xsd:string
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/author"Li L.B."xsd:string
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/author"Zhang W.P."xsd:string
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/author"Liu G.Q."xsd:string
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/author"Ju S.G."xsd:string
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/author"Qian Y.Y."xsd:string
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/author"Lu P.R."xsd:string
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/name"Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/pages"255-259"xsd:string
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/title"[Eukaryotic expression and biological activity of recombinant human IL-17B protein]."xsd:string
http://purl.uniprot.org/citations/22394632http://purl.uniprot.org/core/volume"28"xsd:string
http://purl.uniprot.org/citations/22394632http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/22394632
http://purl.uniprot.org/citations/22394632http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/22394632
http://purl.uniprot.org/uniprot/#_Q6IAG3-mappedCitation-22394632http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22394632
http://purl.uniprot.org/uniprot/#_Q9UHF5-mappedCitation-22394632http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22394632
http://purl.uniprot.org/uniprot/Q9UHF5http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/22394632
http://purl.uniprot.org/uniprot/Q6IAG3http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/22394632