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http://purl.uniprot.org/citations/22396800http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22396800http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22396800http://www.w3.org/2000/01/rdf-schema#comment"Diminishing global fresh water availability has focused research to elucidate mechanisms of water use in poplar, an economically important species. A GT-2 family trihelix transcription factor that is a determinant of water use efficiency (WUE), PtaGTL1 (GT-2 like 1), was identified in Populus tremula × P. alba (clone 717-IB4). Like other GT-2 family members, PtaGTL1 contains both N- and C-terminal trihelix DNA binding domains. PtaGTL1 expression, driven by the Arabidopsis thaliana AtGTL1 promoter, suppressed the higher WUE and drought tolerance phenotypes of an Arabidopsis GTL1 loss-of-function mutation (gtl1-4). Genetic suppression of gtl1-4 was associated with increased stomatal density due to repression of Arabidopsis STOMATAL DENSITY AND DISTRIBUTION1 (AtSDD1), a negative regulator of stomatal development. Electrophoretic mobility shift assays (EMSA) indicated that a PtaGTL1 C-terminal DNA trihelix binding fragment (PtaGTL1-C) interacted with an AtSDD1 promoter fragment containing the GT3 box (GGTAAA), and this GT3 box was necessary for binding. PtaGTL1-C also interacted with a PtaSDD1 promoter fragment via the GT2 box (GGTAAT). PtaSDD1 encodes a protein with 60% primary sequence identity with AtSDD1. In vitro molecular interaction assays were used to determine that Ca(2+)-loaded calmodulin (CaM) binds to PtaGTL1-C, which was predicted to have a CaM-interaction domain in the first helix of the C-terminal trihelix DNA binding domain. These results indicate that, in Arabidopsis and poplar, GTL1 and SDD1 are fundamental components of stomatal lineage. In addition, PtaGTL1 is a Ca(2+)-CaM binding protein, which infers a mechanism by which environmental stimuli can induce Ca(2+) signatures that would modulate stomatal development and regulate plant water use."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0032925"xsd:string
http://purl.uniprot.org/citations/22396800http://purl.org/dc/terms/identifier"doi:10.1371/journal.pone.0032925"xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/author"Hasegawa P.M."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/author"Hasegawa P.M."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/author"Weng H."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/author"Weng H."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/author"Yoo C.Y."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/author"Yoo C.Y."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/author"Mickelbart M.V."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/author"Mickelbart M.V."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/author"Gosney M.J."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/author"Gosney M.J."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/name"PLoS ONE"xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/name"PLoS One"xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/pages"E32925"xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/pages"e32925"xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/title"Poplar GTL1 is a Ca2+/calmodulin-binding transcription factor that functions in plant water use efficiency and drought tolerance."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/title"Poplar GTL1 is a Ca2+/calmodulin-binding transcription factor that functions in plant water use efficiency and drought tolerance."xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/volume"7"xsd:string
http://purl.uniprot.org/citations/22396800http://purl.uniprot.org/core/volume"7"xsd:string
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