| http://purl.uniprot.org/citations/22401818 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22401818 | http://www.w3.org/2000/01/rdf-schema#comment | "The stem cell leukemia (Scl)/Tal1 gene is essential for normal blood and endothelial development, and is expressed in hematopoietic stem cells (HSCs), progenitors, erythroid, megakaryocytic, and mast cells. The Scl +19 enhancer is active in HSCs and progenitor cells, megakaryocytes, and mast cells, but not mature erythroid cells. Here we demonstrate that in vivo deletion of the Scl +19 enhancer (Scl(Δ19/Δ19)) results in viable mice with normal Scl expression in mature hematopoietic lineages. By contrast, Scl expression is reduced in the stem/progenitor compartment and flow cytometry analysis revealed that the HSC and megakaryocyte-erythroid progenitor populations are enlarged in Scl(Δ19/Δ19) mice. The increase in HSC numbers contributed to enhanced expansion in bone marrow transplantation assays, but did not affect multilineage repopulation or stress responses. These results affirm that the Scl +19 enhancer plays a key role in the development of hematopoietic stem/progenitor cells, but is not necessary for mature hematopoietic lineages. Moreover, active histone marks across the Scl locus were significantly reduced in Scl(Δ19/Δ19) fetal liver cells without major changes in steady-state messenger RNA levels, suggesting post-transcriptional compensation for loss of a regulatory element, a result that might be widely relevant given the frequent observation of mild phenotypes after deletion of regulatory elements."xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.exphem.2012.02.006"xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/author | "Green A.R."xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/author | "Ferreira R."xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/author | "Broccardo C."xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/author | "Scott L.M."xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/author | "Gottgens B."xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/author | "Ottersbach K."xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/author | "Spensberger D."xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/author | "Kotsopoulou E."xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/author | "Fourouclas N."xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/name | "Exp Hematol"xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/pages | "588-598.e1"xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/title | "Deletion of the Scl +19 enhancer increases the blood stem cell compartment without affecting the formation of mature blood lineages."xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://purl.uniprot.org/core/volume | "40"xsd:string |
| http://purl.uniprot.org/citations/22401818 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22401818 |
| http://purl.uniprot.org/citations/22401818 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22401818 |
| http://purl.uniprot.org/uniprot/#_A2AD40-mappedCitation-22401818 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22401818 |
| http://purl.uniprot.org/uniprot/#_Q3TZH7-mappedCitation-22401818 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22401818 |
| http://purl.uniprot.org/uniprot/#_P22091-mappedCitation-22401818 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22401818 |
| http://purl.uniprot.org/uniprot/#_Q9JK33-mappedCitation-22401818 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22401818 |
| http://purl.uniprot.org/uniprot/A2AD40 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22401818 |
| http://purl.uniprot.org/uniprot/P22091 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22401818 |