| http://purl.uniprot.org/citations/22402981 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22402981 | http://www.w3.org/2000/01/rdf-schema#comment | "In human intestinal epithelial crypt (HIEC) cells, the PI3-K/Akt-1 pathway is crucial for the promotion of cell survival and suppression of anoikis. Class I PI3-K consists of a complex formed by a catalytic (C) and regulatory (R) subunit. Three R (p85α, β, and p55γ) and four C (p110α, β, γ and δ) isoforms are known. Herein, we analyzed the expression of PI3-K isoforms in HIEC cells and determined their roles in cell survival, as well as in the β1 integrin/Fak/Src-mediated suppression of anoikis. We report that: (1) the predominant PI3-K complexes expressed by HIEC cells are p110α/p85β and p110α/p55γ; (2) the inhibition and/or siRNA-mediated expression silencing of p110α, but not that of p110β, γ or δ, results in Akt-1 down-activation and consequent apoptosis; (3) the expression silencing of p85β or p55γ, but not that of p85α, likewise induces Akt-1 down-activation and apoptosis; however, the impact of a loss of p55γ on both Akt-1 activation and cell survival is significantly greater than that from the loss of p85β; and (4) both the p110α/p85β and p110α/p55γ complexes are engaged by β1 integrin/Fak/Src signaling; however, the engagement of p110α/p85β is primarily Src-dependent, whereas that of p110α/p55γ is primarily Fak-dependent (but Src-independent). Hence, HIEC cells selectively express PI3-K isoform complexes, translating into distinct roles in Akt-1 activation and cell survival, as well as in a selective engagement by Fak and/or Src within the context of β1 integrin/Fak/Src-mediated suppression of anoikis."xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.org/dc/terms/identifier | "doi:10.1007/s10495-012-0713-6"xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/author | "Noel D."xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/author | "Vachon P.H."xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/author | "Beaulieu J.F."xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/author | "Thibodeau S."xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/author | "Bouchard V."xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/author | "Demers M.J."xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/author | "Harnois C."xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/author | "Beausejour M."xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/name | "Apoptosis"xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/pages | "566-578"xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/title | "Integrin/Fak/Src-mediated regulation of cell survival and anoikis in human intestinal epithelial crypt cells: selective engagement and roles of PI3-K isoform complexes."xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://purl.uniprot.org/core/volume | "17"xsd:string |
| http://purl.uniprot.org/citations/22402981 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22402981 |
| http://purl.uniprot.org/citations/22402981 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22402981 |
| http://purl.uniprot.org/uniprot/Q05397#attribution-FCB98D9A026D8742B371AA5B57A5E125 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22402981 |
| http://purl.uniprot.org/uniprot/Q92569#attribution-924E61C9F20F3AE5A56A7B79B3CF23C9 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22402981 |
| http://purl.uniprot.org/uniprot/O00459#attribution-01441304C79C27DF2BC36F80A4B12428 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22402981 |
| http://purl.uniprot.org/uniprot/P42336#attribution-7FC85C4693BA22D9C9608C9201FA4F78 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22402981 |
| http://purl.uniprot.org/uniprot/P42336#attribution-FCB98D9A026D8742B371AA5B57A5E125 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22402981 |
| http://purl.uniprot.org/uniprot/P42338#attribution-01441304C79C27DF2BC36F80A4B12428 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22402981 |
| http://purl.uniprot.org/uniprot/P12931#attribution-FCB98D9A026D8742B371AA5B57A5E125 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22402981 |