| http://purl.uniprot.org/citations/22442681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22442681 | http://www.w3.org/2000/01/rdf-schema#comment | "Yeast is an ideal organism for the development and application of synthetic biology, yet there remain relatively few well-characterised biological parts suitable for precise engineering of this chassis. In order to address this current need, we present here a strategy that takes a single biological part, a promoter, and re-engineers it to produce a fine-graded output range promoter library and new regulated promoters desirable for orthogonal synthetic biology applications. A highly constitutive Saccharomyces cerevisiae promoter, PFY1p, was identified by bioinformatic approaches, characterised in vivo and diversified at its core sequence to create a 36-member promoter library. TetR regulation was introduced into PFY1p to create a synthetic inducible promoter (iPFY1p) that functions in an inverter device. Orthogonal and scalable regulation of synthetic promoters was then demonstrated for the first time using customisable Transcription Activator-Like Effectors (TALEs) modified and designed to act as orthogonal repressors for specific PFY1-based promoters. The ability to diversify a promoter at its core sequences and then independently target Transcription Activator-Like Orthogonal Repressors (TALORs) to virtually any of these sequences shows great promise toward the design and construction of future synthetic gene networks that encode complex "multi-wire" logic functions."xsd:string |
| http://purl.uniprot.org/citations/22442681 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0033279"xsd:string |
| http://purl.uniprot.org/citations/22442681 | http://purl.uniprot.org/core/author | "Ellis T."xsd:string |
| http://purl.uniprot.org/citations/22442681 | http://purl.uniprot.org/core/author | "Blount B.A."xsd:string |
| http://purl.uniprot.org/citations/22442681 | http://purl.uniprot.org/core/author | "Vasylechko S."xsd:string |
| http://purl.uniprot.org/citations/22442681 | http://purl.uniprot.org/core/author | "Weenink T."xsd:string |
| http://purl.uniprot.org/citations/22442681 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22442681 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/22442681 | http://purl.uniprot.org/core/pages | "e33279"xsd:string |
| http://purl.uniprot.org/citations/22442681 | http://purl.uniprot.org/core/title | "Rational diversification of a promoter providing fine-tuned expression and orthogonal regulation for synthetic biology."xsd:string |
| http://purl.uniprot.org/citations/22442681 | http://purl.uniprot.org/core/volume | "7"xsd:string |
| http://purl.uniprot.org/citations/22442681 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22442681 |
| http://purl.uniprot.org/citations/22442681 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22442681 |
| http://purl.uniprot.org/uniprot/#_P08456-mappedCitation-22442681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22442681 |
| http://purl.uniprot.org/uniprot/#_Q01976-mappedCitation-22442681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22442681 |
| http://purl.uniprot.org/uniprot/#_P38115-mappedCitation-22442681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22442681 |
| http://purl.uniprot.org/uniprot/#_P40825-mappedCitation-22442681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22442681 |
| http://purl.uniprot.org/uniprot/#_Q08273-mappedCitation-22442681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22442681 |
| http://purl.uniprot.org/uniprot/#_P00044-mappedCitation-22442681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22442681 |
| http://purl.uniprot.org/uniprot/#_P00330-mappedCitation-22442681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22442681 |
| http://purl.uniprot.org/uniprot/#_P08679-mappedCitation-22442681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22442681 |
| http://purl.uniprot.org/uniprot/#_P07274-mappedCitation-22442681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22442681 |
| http://purl.uniprot.org/uniprot/#_P32419-mappedCitation-22442681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22442681 |
| http://purl.uniprot.org/uniprot/#_P32451-mappedCitation-22442681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22442681 |