| http://purl.uniprot.org/citations/22446793 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22446793 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22446793 | http://www.w3.org/2000/01/rdf-schema#comment | "Carboxylesterase 2 (CES2), the main carboxylesterase expressed in human intestine, is an increasingly important enzyme in anti-cancer combined therapies for the treatment of different pathologies like colon adenocarcinoma and malignant glioma. The production of human recombinant CES2, in human embryonic kidney cells (HEK-293T cells) using serum-free media, is herein described. CES2 secretion to the media was achieved by the simple addition of an in-frame C-terminal 10× histidine tag (CES2-10xHis) without the need of addition of extra N-terminal signalling sequences or the mutation or deletion of the C-terminal HTEL motif responsible for retaining the protein in the lumen of endoplasmic reticulum. This secretion allowed a fourfold increase in CES2 production. The characterization of human recombinant CES2 showed that this protein exists in other active and inactive forms than the described 60 kDa monomer."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00253-012-3994-3"xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.org/dc/terms/identifier | "doi:10.1007/s00253-012-3994-3"xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Peixoto C."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Peixoto C."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Alves P.M."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Alves P.M."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Coroadinha A.S."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Coroadinha A.S."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Cunha B."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Cunha B."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Lamego J."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Lamego J."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Simplicio A.L."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Simplicio A.L."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Sousa M.F."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/author | "Sousa M.F."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/date | "2013"xsd:gYear |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/name | "Appl. Microbiol. Biotechnol."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/name | "Appl. Microbiol. Biotechnol."xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/pages | "1161-1173"xsd:string |
| http://purl.uniprot.org/citations/22446793 | http://purl.uniprot.org/core/pages | "1161-1173"xsd:string |