| http://purl.uniprot.org/citations/22451653 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22451653 | http://www.w3.org/2000/01/rdf-schema#comment | "Osteoclasts are multinucleated giant cells that reside in osseous tissues and resorb bone. Signaling mediated by receptor activator of nuclear factor (NF)-κB (RANK) and its ligand leads to the nuclear factor of activated T cells 2/c1 (NFAT2 or NFATc1) expression, a critical step in the formation of functional osteoclasts. In addition, adaptor proteins harboring immunoreceptor tyrosine-based activation motifs, such as DNAX-activating protein of 12 kDa (DAP12), play essential roles. In this study, we identified the gene encoding the lectin Siglec-15 as NFAT2-inducible, and we found that the protein product links RANK ligand-RANK-NFAT2 and DAP12 signaling in mouse osteoclasts. Both the recognition of sialylated glycans by the Siglec-15 V-set domain and the association with DAP12 through its Lys-272 are essential for its function. When Siglec-15 expression was knocked down, fewer multinucleated cells developed, and those that did were morphologically contracted with disordered actin-ring structures. These changes were accompanied by significantly reduced bone resorption. Siglec-15 formed complexes with Syk through DAP12 in response to vitronectin. Furthermore, chimeric molecules consisting of the extracellular and transmembrane regions of Siglec-15 with a K272A mutation and the cytoplasmic region of DAP12 significantly restored bone resorption in cells with knocked down Siglec-15 expression. Together, these results suggested that the Siglec-15-DAP12-Syk-signaling cascade plays a critical role in functional osteoclast formation."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.324194"xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/author | "Tanaka K."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/author | "Hayashi K."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/author | "Kimura T."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/author | "Miura T."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/author | "Ogawa T."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/author | "Sato M."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/author | "Maruoka M."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/author | "Bao X."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/author | "Takeya T."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/author | "Miyoshi J."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/author | "Kitaoka Y."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/author | "Ishida-Kitagawa N."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/pages | "17493-17502"xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/title | "Siglec-15 protein regulates formation of functional osteoclasts in concert with DNAX-activating protein of 12 kDa (DAP12)."xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://purl.uniprot.org/core/volume | "287"xsd:string |
| http://purl.uniprot.org/citations/22451653 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22451653 |
| http://purl.uniprot.org/citations/22451653 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22451653 |
| http://purl.uniprot.org/uniprot/P07141#attribution-26FB809A8867CE004B9E5A7767C80363 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22451653 |
| http://purl.uniprot.org/uniprot/P09581#attribution-26FB809A8867CE004B9E5A7767C80363 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22451653 |
| http://purl.uniprot.org/uniprot/P48025#attribution-26FB809A8867CE004B9E5A7767C80363 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/22451653 |