| http://purl.uniprot.org/citations/22451656 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22451656 | http://www.w3.org/2000/01/rdf-schema#comment | "The epithelial-to-mesenchymal transition (EMT) plays crucial roles in embryonic development, wound healing, tissue repair, and cancer progression. Results of this study show how transforming growth factor β1 (TGF-β1) down-regulates expression of N-acetylglucosaminyltransferase III (GnT-III) during EMT-like changes. Treatment with TGF-β1 resulted in a decrease in E-cadherin expression and GnT-III expression, as well as its product, the bisected N-glycans, which was confirmed by erythro-agglutinating phytohemagglutinin lectin blot and HPLC analysis in human MCF-10A and mouse GE11 cells. In contrast with GnT-III, the expression of N-acetylglucosaminyltransferase V was slightly enhanced by TGF-β1 treatment. Changes in the N-glycan patterns on α3β1 integrin, one of the target proteins for GnT-III, were also confirmed by lectin blot analysis. To understand the roles of GnT-III expression in EMT-like changes, the MCF-10A cell was stably transfected with GnT-III. It is of particular interest that overexpression of GnT-III influenced EMT-like changes induced by TGF-β1, which was confirmed by cell morphological changes of phase contrast, immunochemical staining patterns of E-cadherin, and actin. In addition, GnT-III modified E-cadherin, which served to prolong E-cadherin turnover on the cell surface examined by biotinylation and pulse-chase experiments. GnT-III expression consistently inhibited β-catenin translocation from cell-cell contact into the cytoplasm and nucleus. Furthermore, the transwell assay showed that GnT-III expression suppressed TGF-β1-induced cell motility. Taken together, these observations are the first to clearly demonstrate that GnT-III affects cell properties, which in turn influence EMT-like changes, and to explain a molecular mechanism for the inhibitory effects of GnT-III on cancer metastasis."xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.262154"xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/author | "Du Y."xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/author | "Gu W."xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/author | "Gu J."xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/author | "Kondo M."xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/author | "Fukuda T."xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/author | "Lu Y."xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/author | "Xu Q."xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/author | "Isaji T."xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/pages | "16563-16574"xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/title | "Roles of N-acetylglucosaminyltransferase III in epithelial-to-mesenchymal transition induced by transforming growth factor beta1 (TGF-beta1) in epithelial cell lines."xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://purl.uniprot.org/core/volume | "287"xsd:string |
| http://purl.uniprot.org/citations/22451656 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22451656 |
| http://purl.uniprot.org/citations/22451656 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22451656 |
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