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http://purl.uniprot.org/citations/22465949http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22465949http://www.w3.org/2000/01/rdf-schema#comment"

Aim

To investigate the influences of breviscapine, a flavonoid extracted from Erigeron breviscapus, on the proliferation and migration of vascular smooth muscle cells (VSMCs) cultured in a high glucose medium and the underlying mechanisms.

Methods

VSMCs were isolated from thoracic aortas of male Sprague-Dawley rats and cultured in vitro. Cell proliferation was evaluated using Counting Kit-8 cell viability assay. Cell migration was evaluated using transwell migration assay and in vitro scratch assay. The expression and activity of protein kinase C-β2 (PKC-β2), extracellular signal-regulated kinase 1/2 (ERK1/2), p38 mitogen-activated protein kinase (p38), and JNK mitogen-activated protein kinase (JNK) were measured with Western blotting.

Results

Exposure of VSMCs to a high glucose (25 mmol/L) medium significantly increased the proliferation and migration potential as compared to the control group. Pretreatment with breviscapine (65 μmol/L and 108 μmol/L) attenuated high glucose-enhanced proliferation and migration of VSMCs. Exposure of VSMCs to the high glucose medium activated both the PKC-β2 and ERK1/2 MAPK, but not the p38 and JNK MAPK. Pretreatment with breviscapine (65 μmol/L and 108 μmol/L) blocked high glucose-induced increase of the ERK1/2 activity, but not that of the PKC-β2 activity.

Conclusion

Our study demonstrated that breviscapine ameliorates high glucose-induced proliferation and migration of VSMCs via inhibiting ERK1/2 MAPK signaling."xsd:string
http://purl.uniprot.org/citations/22465949http://purl.org/dc/terms/identifier"doi:10.1038/aps.2012.6"xsd:string
http://purl.uniprot.org/citations/22465949http://purl.uniprot.org/core/author"He M."xsd:string
http://purl.uniprot.org/citations/22465949http://purl.uniprot.org/core/author"Li J."xsd:string
http://purl.uniprot.org/citations/22465949http://purl.uniprot.org/core/author"Zhou B.Q."xsd:string
http://purl.uniprot.org/citations/22465949http://purl.uniprot.org/core/author"Xue Z.M."xsd:string
http://purl.uniprot.org/citations/22465949http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22465949http://purl.uniprot.org/core/name"Acta Pharmacol Sin"xsd:string
http://purl.uniprot.org/citations/22465949http://purl.uniprot.org/core/pages"606-614"xsd:string
http://purl.uniprot.org/citations/22465949http://purl.uniprot.org/core/title"Breviscapine inhibits high glucose-induced proliferation and migration of cultured vascular smooth muscle cells of rats via suppressing the ERK1/2 MAPK signaling pathway."xsd:string
http://purl.uniprot.org/citations/22465949http://purl.uniprot.org/core/volume"33"xsd:string
http://purl.uniprot.org/citations/22465949http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/22465949
http://purl.uniprot.org/citations/22465949http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/22465949
http://purl.uniprot.org/uniprot/#_P63086-mappedCitation-22465949http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22465949
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http://purl.uniprot.org/uniprot/#_P21708-mappedCitation-22465949http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22465949
http://purl.uniprot.org/uniprot/A6JSN9http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/22465949
http://purl.uniprot.org/uniprot/P63086http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/22465949
http://purl.uniprot.org/uniprot/P21708http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/22465949