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Background

We have identified syndecan-2 as a protein potentially involved in perineural invasion of pancreatic adenocarcinoma (PDAC) cells.

Methods

Syndecan-2 (SDC-2) expression was analyzed in human normal pancreas, chronic pancreatitis and PDAC tissues. Functional in vitro assays were carried out to determine its role in invasion, migration and signaling.

Results

SDC-2 was expressed in the majority of the tested pancreatic cancer cell lines while it was upregulated in nerve-invasive PDAC cell clones. There were 2 distinct expression patterns of SDC-2 in PDAC tissue samples: SDC-2 positivity in the cancer cell cytoplasm and a peritumoral expression. Though SDC-2 silencing (using specific siRNA oligonucleotides) did not affect anchorage-dependent growth, it significantly reduced cell motility and invasiveness in the pancreatic cancer cell lines T3M4 and Su8686. On the transcriptional level, migration-and invasion-associated genes were down-regulated following SDC-2 RNAi. Furthermore, SDC-2 silencing reduced K-ras activity, phosphorylation of Src and--further downstream--phosphorylation of ERK2 while levels of the putative SDC-2 signal transducer p120GAP remained unaltered.

Conclusion

SDC-2 is a novel (perineural) invasion-associated gene in PDAC which cooperates with K-ras to induce a more invasive phenotype."xsd:string
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http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/author"Friess H."xsd:string
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/author"Michalski C.W."xsd:string
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/author"Erkan M."xsd:string
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/author"Kleeff J."xsd:string
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/author"Kong B."xsd:string
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/author"De Oliveira T."xsd:string
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/author"Abiatari I."xsd:string
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/author"Raulefs S."xsd:string
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/author"Sauliunaite D."xsd:string
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/name"Mol Cancer"xsd:string
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/pages"19"xsd:string
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/title"Syndecan-2 promotes perineural invasion and cooperates with K-ras to induce an invasive pancreatic cancer cell phenotype."xsd:string
http://purl.uniprot.org/citations/22471946http://purl.uniprot.org/core/volume"11"xsd:string
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