| http://purl.uniprot.org/citations/22472440 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22472440 | http://www.w3.org/2000/01/rdf-schema#comment | "γ-Tubulin complexes are essential for microtubule (MT) nucleation. The γ-tubulin small complex (γ-TuSC) consists of two molecules of γ-tubulin and one molecule each of Spc97 and Spc98. In vitro, γ-TuSCs oligomerize into spirals of 13 γ-tubulin molecules per turn. However, the properties and numbers of γ-TuSCs at MT nucleation sites in vivo are unclear. In this paper, we show by fluorescence recovery after photobleaching analysis that γ-tubulin was stably integrated into MT nucleation sites and was further stabilized by tubulin binding. Importantly, tubulin showed a stronger interaction with the nucleation site than with the MT plus end, which probably provides the basis for MT nucleation. Quantitative analysis of γ-TuSCs on single MT minus ends argued for nucleation sites consisting of approximately seven γ-TuSCs with approximately three additional γ-tubulin molecules. Nucleation and anchoring of MTs required the same number of γ-tubulin molecules. We suggest that a spiral of seven γ-TuSCs with a slight surplus of γ-tubulin nucleates MTs in vivo."xsd:string |
| http://purl.uniprot.org/citations/22472440 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.201111123"xsd:string |
| http://purl.uniprot.org/citations/22472440 | http://purl.uniprot.org/core/author | "Schiebel E."xsd:string |
| http://purl.uniprot.org/citations/22472440 | http://purl.uniprot.org/core/author | "Antony C."xsd:string |
| http://purl.uniprot.org/citations/22472440 | http://purl.uniprot.org/core/author | "Neuner A."xsd:string |
| http://purl.uniprot.org/citations/22472440 | http://purl.uniprot.org/core/author | "Gombos L."xsd:string |
| http://purl.uniprot.org/citations/22472440 | http://purl.uniprot.org/core/author | "Erlemann S."xsd:string |
| http://purl.uniprot.org/citations/22472440 | http://purl.uniprot.org/core/author | "Gibeaux R."xsd:string |
| http://purl.uniprot.org/citations/22472440 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22472440 | http://purl.uniprot.org/core/name | "J Cell Biol"xsd:string |
| http://purl.uniprot.org/citations/22472440 | http://purl.uniprot.org/core/pages | "59-74"xsd:string |
| http://purl.uniprot.org/citations/22472440 | http://purl.uniprot.org/core/title | "An extended gamma-tubulin ring functions as a stable platform in microtubule nucleation."xsd:string |
| http://purl.uniprot.org/citations/22472440 | http://purl.uniprot.org/core/volume | "197"xsd:string |
| http://purl.uniprot.org/citations/22472440 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22472440 |
| http://purl.uniprot.org/citations/22472440 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22472440 |
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