| http://purl.uniprot.org/citations/22496420 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/22496420 | http://www.w3.org/2000/01/rdf-schema#comment | "The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef directs virus escape from immune surveillance by subverting host cell intracellular signaling and membrane traffic to down-regulate cell-surface major histocompatibility complex class I (MHC-I). The interaction of Nef with the sorting proteins PACS-1 and PACS-2 mediates key signaling and trafficking steps required for Nef-mediated MHC-I down-regulation. Little is known, however, about the molecular basis underlying the Nef-PACS interaction. Here we identify the sites on Nef and the PACS proteins required for their interaction and describe the consequences of disrupting this interaction for Nef action. A previously unidentified cargo subsite on PACS-1 and PACS-2 interacted with a bipartite site on Nef formed by the EEEE(65) acidic cluster on the N-terminal domain and W(113) in the core domain. Mutation of these sites prevented the interaction between Nef and the PACS proteins on Rab5 (PACS-2 and PACS-1)- or Rab7 (PACS-1)-positive endosomes as determined by bimolecular fluorescence complementation and caused a Nef mutant defective in PACS binding to localize to distorted endosomal compartments. Consequently, disruption of the Nef-PACS interaction repressed Nef-induced MHC-I down-regulation in peripheral blood mononuclear cells. Our results provide insight into the molecular basis of Nef action and suggest new strategies to combat HIV-1."xsd:string |
| http://purl.uniprot.org/citations/22496420 | http://purl.org/dc/terms/identifier | "doi:10.1091/mbc.e11-11-0928"xsd:string |
| http://purl.uniprot.org/citations/22496420 | http://purl.uniprot.org/core/author | "Thomas L."xsd:string |
| http://purl.uniprot.org/citations/22496420 | http://purl.uniprot.org/core/author | "Thomas G."xsd:string |
| http://purl.uniprot.org/citations/22496420 | http://purl.uniprot.org/core/author | "Dikeakos J.D."xsd:string |
| http://purl.uniprot.org/citations/22496420 | http://purl.uniprot.org/core/author | "Shinde U."xsd:string |
| http://purl.uniprot.org/citations/22496420 | http://purl.uniprot.org/core/author | "Elferich J."xsd:string |
| http://purl.uniprot.org/citations/22496420 | http://purl.uniprot.org/core/author | "Kwon G."xsd:string |
| http://purl.uniprot.org/citations/22496420 | http://purl.uniprot.org/core/date | "2012"xsd:gYear |
| http://purl.uniprot.org/citations/22496420 | http://purl.uniprot.org/core/name | "Mol Biol Cell"xsd:string |
| http://purl.uniprot.org/citations/22496420 | http://purl.uniprot.org/core/pages | "2184-2197"xsd:string |
| http://purl.uniprot.org/citations/22496420 | http://purl.uniprot.org/core/title | "An interdomain binding site on HIV-1 Nef interacts with PACS-1 and PACS-2 on endosomes to down-regulate MHC-I."xsd:string |
| http://purl.uniprot.org/citations/22496420 | http://purl.uniprot.org/core/volume | "23"xsd:string |
| http://purl.uniprot.org/citations/22496420 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/22496420 |
| http://purl.uniprot.org/citations/22496420 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/22496420 |
| http://purl.uniprot.org/uniprot/#_P04601-mappedCitation-22496420 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22496420 |
| http://purl.uniprot.org/uniprot/#_Q8N7J0-mappedCitation-22496420 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22496420 |
| http://purl.uniprot.org/uniprot/#_Q8TDX4-mappedCitation-22496420 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22496420 |
| http://purl.uniprot.org/uniprot/#_Q8TDZ8-mappedCitation-22496420 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22496420 |
| http://purl.uniprot.org/uniprot/#_Q86VP3-mappedCitation-22496420 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/22496420 |
| http://purl.uniprot.org/uniprot/Q8TDX4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22496420 |
| http://purl.uniprot.org/uniprot/P04601 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22496420 |
| http://purl.uniprot.org/uniprot/Q86VP3 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22496420 |
| http://purl.uniprot.org/uniprot/Q8N7J0 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/22496420 |