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http://purl.uniprot.org/citations/22498774http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/22498774http://www.w3.org/2000/01/rdf-schema#comment"Phytochrome A (phyA) is the dominant photoreceptor of far-red light sensing in Arabidopsis thaliana. phyA accumulates at high levels in the cytoplasm of etiolated seedlings, and light-induced phyA signaling is mediated by a complex regulatory network. This includes light- and FHY1/FHL protein-dependent translocation of native phyA into the nucleus in vivo. It has also been shown that a short N-terminal fragment of phyA (PHYA406) is sufficient to phenocopy this highly regulated cellular process in vitro. To test the biological activity of this N-terminal fragment of phyA in planta, we produced transgenic phyA-201 plants expressing the PHYA406-YFP (YELLOW FLUORESCENT PROTEIN)-DD, PHYA406-YFP-DD-NLS (nuclear localization signal), and PHYA406-YFP-DD-NES (nuclear export signal) fusion proteins. Here, we report that PHYA406-YFP-DD is imported into the nucleus and this process is partially light-dependent whereas PHYA406-YFP-DD-NLS and PHYA406-YFP-DD-NES display the expected constitutive localization patterns. Our results show that these truncated phyA proteins are light-stable, they trigger a constitutive photomorphogenic-like response when localized in the nuclei, and neither of them induces proper phyA signaling. We demonstrate that in vitro and in vivo PHYA406 Pfr and Pr bind COP1, a general repressor of photomorphogenesis, and co-localize with it in nuclear bodies. Thus, we conclude that, in planta, the truncated PHYA406 proteins inactivate COP1 in the nuclei in a light-independent fashion."xsd:string
http://purl.uniprot.org/citations/22498774http://purl.org/dc/terms/identifier"doi:10.1093/mp/sss035"xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/author"Heijde M."xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/author"Adam E."xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/author"Nagy F."xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/author"Ulm R."xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/author"Viczian A."xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/author"Kircher S."xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/author"Wolf I."xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/author"Schafer E."xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/author"Bindics J."xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/date"2012"xsd:gYear
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/name"Mol Plant"xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/pages"629-641"xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/title"A short amino-terminal part of Arabidopsis phytochrome A induces constitutive photomorphogenic response."xsd:string
http://purl.uniprot.org/citations/22498774http://purl.uniprot.org/core/volume"5"xsd:string
http://purl.uniprot.org/citations/22498774http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/22498774
http://purl.uniprot.org/citations/22498774http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/22498774
http://purl.uniprot.org/uniprot/#_A0A1P8AVD9-mappedCitation-22498774http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22498774
http://purl.uniprot.org/uniprot/#_A0A178W883-mappedCitation-22498774http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22498774
http://purl.uniprot.org/uniprot/#_A0A1P8B2S1-mappedCitation-22498774http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22498774
http://purl.uniprot.org/uniprot/#_A8MR65-mappedCitation-22498774http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22498774
http://purl.uniprot.org/uniprot/#_P14712-mappedCitation-22498774http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22498774
http://purl.uniprot.org/uniprot/#_P43254-mappedCitation-22498774http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/22498774